X-Ray Diffraction data from human WDR5 in complex with MLL Win6mer peptide, source of 5SXM structure

Data DOI: 10.15785/SBGRID/447 | ID: 447

Publication DOI: 10.1074/jbc.M116.752626

5SXM Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Cosgrove Laboratory, SUNY Upstate Medical University

Release Date: May 26, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/447

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/447 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/447 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/447 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/447 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 447 ; shasum -c files.sha'

Storage requirements: 2.2G

Biological Sample:

human WDR5 in complex with MLL Win6mer peptide

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

MacCHESS F1

Data Creation Date:

Nov. 26, 2014

Related Datasets:

None


Cite this Dataset

Alicea-Velazquez, NL; Shinsky, SA; Cosgrove, M. 2017. "X-Ray Diffraction data for: human WDR5 in complex with MLL Win6mer peptide. PDB Code 5SXM", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/447.

Download Citation

Dataset Description

Native data set

Project Members

Name Additional Roles Affiliation While Working on the Project
Nilda L Alicea-VelazquezData Collector, DepositorSUNY Upstate Medical University
Stephen A ShinskyData CollectorSUNY Upstate Medical University
Michael CosgrovePISUNY Upstate Medical University

Reprocessing Instructions

Data indexed to P31 with HKL2000 at 1.8 A resolution, then rescaled to 2.0 A resolution.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9773

Sweep:

SWEEP1

Files:

/programs/datagrid/447/G78_1_###.img

Images:

1 - 270

Beam:

(137.36, 124.17) -> (137.36, 124.13)

Distance:

250.0 -> 251.56

Spacegroup:

P 31 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.73 4.7 1.73
Low resolution limit 53.32 53.34 1.76
Completeness 98.7 100.0 85.3
Multiplicity 13.6 15.2 5.7
I/sigma 13.7 41.5 1.0
Rmerge 0.116 0.052 1.446
CC half 0.999 0.999 0.426
Anomalous completeness 97.9 99.9 81.4
Anomalous multiplicity 7.2 7.2 7.2
Cell 74.94 74.94 93.51 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9773

Sweep:

SWEEP1

Files:

/programs/datagrid/447/G78_1_###.img

Images:

1 - 270

Beam:

(137.36, 124.17) -> (137.36, 124.13)

Distance:

250.0 -> 251.63

Spacegroup:

P 31 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.74 4.72 1.74
Low resolution limit 53.32 53.34 1.77
Completeness 98.9 100.0 88.2
Multiplicity 13.8 15.2 5.8
I/sigma 13.9 41.6 1.0
Rmerge 0.116 0.052 1.47
CC half 0.999 0.999 0.425
Anomalous completeness 98.3 99.9 84.5
Anomalous multiplicity 7.3 7.3 7.3
Cell 74.94 74.94 93.51 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9773

Sweep:

SWEEP1

Files:

/programs/datagrid/447/G78_1_###.img

Images:

1 - 270

Beam:

(137.36, 124.17) -> (137.36, 124.13)

Distance:

250.0 -> 251.87

Spacegroup:

P 31 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.71 4.64 1.71
Low resolution limit 53.42 53.44 1.74
Completeness 97.7 100.0 79.4
Multiplicity 13.1 15.1 5.0
I/sigma 11.0 26.7 1.0
Rmerge 0.128 0.068 1.112
CC half 0.998 0.998 0.526
Anomalous completeness 96.7 100.0 73.8
Anomalous multiplicity 6.9 6.9 6.9
Cell 75.09 75.09 93.658 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.