X-Ray Diffraction data from Rif1 N-terminal domain (RIF1-NTD) from Saccharomyces cerevisiae in complex with DNA, source of 5NW5 structure

Data DOI: 10.15785/SBGRID/446 | ID: 446

Publication DOI: 10.1038/nsmb.3420

5NW5 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Thoma Laboratory, Friedrich Miescher Institute for Biomedical Research

Release Date: June 13, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/446

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/446 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/446 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/446 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/446 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 446 ; shasum -c files.sha'

Storage requirements: 13G

Biological Sample:

Rif1 N-terminal domain (RIF1-NTD) from Saccharomyces cerevisiae in complex with DNA

Dataset Type:

X-Ray Diffraction

Subject Composition:

DNA, Protein

Collection Facility:

Beamline PXII (X10SA), Swiss Light Source, Switzerland

Data Creation Date:

Feb. 26, 2014

Related Datasets:

None


Cite this Dataset

Bunker, RD; Thoma, N. 2017. "X-Ray Diffraction data for: Rif1 N-terminal domain (RIF1-NTD) from Saccharomyces cerevisiae in complex with DNA. PDB Code 5NW5", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/446.

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Dataset Description

Three sweeps combined for final data set. Sweep 1 collected from a separate position on the crystal to subsequence sweeps. Sweeps 2 and 3 collected in inverse beam mode

Project Members

Name Additional Roles Affiliation While Working on the Project
Richard D BunkerData Collector, DepositorFriedrich Miescher Institute for Biomedical Research
Nicolas ThomaPIhttps://www.fmi.ch/research/groupleader/members/?group=118

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.91863

Sweep:

SWEEP3

Files:

/programs/datagrid/446/5NW5_3_#####.cbf

Images:

1 - 1800

Beam:

(146.72, 119.02) -> (146.72, 119.02)

Distance:

800.02 -> 799.38

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 8.05 21.63 8.05
Low resolution limit 68.72 68.73 8.19
Completeness 99.6 100.0 97.8
Multiplicity 10.2 12.0 6.5
I/sigma 11.7 30.6 1.4
Rmerge 0.131 0.061 1.177
CC half 0.997 0.998 0.742
Anomalous completeness 98.9 97.3 97.8
Anomalous multiplicity 5.7 5.7 5.7
Cell 92.12 169.71 390.0 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.91863

Sweep:

SWEEP3

Files:

/programs/datagrid/446/5NW5_3_#####.cbf

Images:

1 - 1800

Beam:

(146.72, 119.02) -> (146.72, 119.04)

Distance:

800.02 -> 800.84

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 10.05 26.91 10.05
Low resolution limit 78.13 78.13 10.22
Completeness 100.0 100.0 97.2
Multiplicity 16.2 11.8 5.9
I/sigma 9.8 9.0 4.9
Rmerge 0.166 0.098 0.136
CC half 0.996 0.997 0.994
Anomalous completeness 99.7 96.2 96.6
Anomalous multiplicity 9.2 9.2 9.2
Cell 92.278 169.926 390.646 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.