X-Ray Diffraction data from Single-Domain Antibody F-VHH-4, source of 5TP3 structure

Data DOI: 10.15785/SBGRID/426 | ID: 426

Publication DOI: 10.1038/ncomms14158

5TP3 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

McLellan Laboratory, University of Texas at Austin

Release Date: March 3, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/426

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/426 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/426 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/426 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/426 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 426 ; shasum -c files.sha'

Storage requirements: 6.4G

Biological Sample:

Single-Domain Antibody F-VHH-4

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

19-ID, SBC, APS, Chicago, IL

Data Creation Date:

Nov. 3, 2015

Related Datasets:

None


Cite this Dataset

Wrapp, D; McLellan, J. 2017. "X-Ray Diffraction data for: Single-Domain Antibody F-VHH-4. PDB Code 5TP3", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/426.

Download Citation

Dataset Description

Crystal structure of the RSV-neutralizing single-domain antibody F-VHH-4

Project Members

Name Additional Roles Affiliation While Working on the Project
Daniel WrappData Collector, DepositorDartmouth College
Jason McLellanPIUniversity of Texas at Austin

Reprocessing Instructions

Beam center needs to be corrected to X=153.3 Y=161.1 Processing with iMosflm will require "Reverse direction of spindle rotation" to be checked.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97929

Sweep:

SWEEP1

Files:

/programs/datagrid/426/Nb4_X2.####.img

Images:

1 - 360

Beam:

(154.5, 159.98) -> (154.5, 161.16)

Distance:

274.39 -> 272.74

Spacegroup:

P 42 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.81 4.91 1.81
Low resolution limit 30.01 30.01 1.84
Completeness 100.0 100.0 100.0
Multiplicity 13.1 11.6 9.0
I/sigma 14.3 27.5 1.2
Rmerge 0.098 0.065 1.807
CC half 0.998 0.998 0.344
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 7.3 7.3 7.3
Cell 47.81 47.81 150.05 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97929

Sweep:

SWEEP1

Files:

/programs/datagrid/426/Nb4_X2.####.img

Images:

1 - 360

Beam:

(154.5, 159.98) -> (154.5, 161.17)

Distance:

274.39 -> 274.18

Spacegroup:

P 42 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.81 4.91 1.81
Low resolution limit 45.54 45.56 1.84
Completeness 100.0 100.0 100.0
Multiplicity 13.1 11.6 9.0
I/sigma 14.3 27.5 1.2
Rmerge 0.098 0.065 1.816
CC half 0.998 0.997 0.354
Anomalous completeness 100.0 100.0 99.9
Anomalous multiplicity 7.3 7.3 7.3
Cell 47.8 47.8 150.04 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97929

Sweep:

SWEEP1

Files:

/programs/datagrid/426/Nb4_X2.####.img

Images:

1 - 360

Beam:

(154.5, 159.98) -> (154.5, 161.18)

Distance:

274.39 -> 271.35

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.82 4.94 1.82
Low resolution limit 45.27 45.28 1.85
Completeness 100.0 100.0 100.0
Multiplicity 13.1 11.6 9.2
I/sigma 11.1 20.1 1.3
Rmerge 0.136 0.076 4.217
CC half 0.997 0.997 0.394
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 7.3 7.3 7.3
Cell 47.511 47.511 149.049 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.