X-Ray Diffraction data from Frizzled-8 complex with designed Wnt agonist, source of 5UN5 structure

Data DOI: 10.15785/SBGRID/423 | ID: 423

Publication DOI: 10.1038/NATURE22306

5UN5 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: 7 Nov 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/423

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/423 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/423 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/423 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/423 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 423 ; shasum -c files.sha'

Storage requirements: 3.6G

Biological Sample:

Frizzled-8 complex with designed Wnt agonist

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ALS 8.2.2

Data Creation Date:

22 Sep 2013

Related Datasets:

None


Cite this Dataset

Jude, KM.; Garcia, KC.; Janda, CY.; Garcia, K. 2017. "X-Ray Diffraction data for: Frizzled-8 complex with designed Wnt agonist. PDB Code 5UN5", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/423.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M. JudeDepositorHoward Hughes Medical Institute
K. C. GarciaStanford University/Howard Hughes Medical Institute
Claudia Y. JandaData CollectorStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99997

Sweep:

SWEEP1

Files:

/programs/datagrid/423/C14_8_###.img

Images:

1 - 200

Beam:

(155.02, 163.45) -> (155.02, 163.15)

Distance:

450.0 -> 460.84

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.05 8.26 3.05
Low resolution limit 83.01 83.04 3.1
Completeness 99.9 100.0 95.3
Multiplicity 4.2 4.0 3.8
I/sigma 9.3 48.5 0.3
Rmerge 0.145 0.037 63.979
CC half 0.971 0.992 0.38
Anomalous completeness 99.6 100.0 93.7
Anomalous multiplicity 2.2 2.2 2.2
Cell 42.108 79.151 83.116 90.0 92.843 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.