X-Ray Diffraction data from Frizzled-8 complex with designed Wnt agonist, source of 5UN6 structure

Data DOI: 10.15785/SBGRID/422 | ID: 422

Publication DOI: 10.1038/NATURE22306

5UN6 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: Nov. 7, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/422

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/422 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/422 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/422 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/422 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 422 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Frizzled-8 complex with designed Wnt agonist

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ALS 8.2.2

Data Creation Date:

Sept. 21, 2013

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Janda, CY; Garcia, K. 2017. "X-Ray Diffraction data for: Frizzled-8 complex with designed Wnt agonist. PDB Code 5UN6", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/422.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeDepositorHoward Hughes Medical Institute
K. C GarciaStanford University/Howard Hughes Medical Institute
Claudia Y JandaData CollectorStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

processed with XDS

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99997

Sweep:

SWEEP1

Files:

/programs/datagrid/422/A1_3_###.img

Images:

1 - 180

Beam:

(155.02, 163.45) -> (155.02, 163.1)

Distance:

450.0 -> 450.61

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.18 8.63 3.18
Low resolution limit 116.4 116.48 3.23
Completeness 100.0 100.0 100.0
Multiplicity 3.5 3.2 3.6
I/sigma 5.2 11.4 1.4
Rmerge 0.165 0.041 1.146
CC half 0.985 0.998 0.357
Anomalous completeness 98.7 100.0 99.4
Anomalous multiplicity 1.9 1.9 1.9
Cell 116.647 36.521 125.811 90.0 93.698 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99997

Sweep:

SWEEP1

Files:

/programs/datagrid/422/A1_3_###.img

Images:

1 - 180

Beam:

(155.02, 163.45) -> (155.02, 163.06)

Distance:

450.0 -> 449.61

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.98 8.08 2.98
Low resolution limit 62.64 62.65 3.03
Completeness 98.2 92.9 99.5
Multiplicity 3.6 3.2 3.5
I/sigma 8.4 25.5 1.1
Rmerge 0.111 0.024 1.125
CC half 0.996 0.999 0.408
Anomalous completeness 94.0 85.2 97.9
Anomalous multiplicity 1.9 1.9 1.9
Cell 116.42 36.44 125.54 90.0 93.74 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99997

Sweep:

SWEEP1

Files:

/programs/datagrid/422/A1_3_###.img

Images:

1 - 180

Beam:

(155.02, 163.45) -> (155.02, 163.06)

Distance:

450.0 -> 449.26

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.99 8.11 2.99
Low resolution limit 62.64 62.65 3.04
Completeness 98.2 93.1 99.4
Multiplicity 3.6 3.2 3.6
I/sigma 8.4 25.4 1.1
Rmerge 0.11 0.024 1.079
CC half 0.996 0.999 0.476
Anomalous completeness 93.8 85.3 97.7
Anomalous multiplicity 1.9 1.9 1.9
Cell 116.42 36.44 125.54 90.0 93.74 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99997

Sweep:

SWEEP1

Files:

/programs/datagrid/422/A1_3_###.img

Images:

1 - 180

Beam:

(155.02, 163.45) -> (155.02, 163.05)

Distance:

450.0 -> 445.76

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.06 8.31 3.06
Low resolution limit 115.75 115.84 3.11
Completeness 99.5 100.0 89.8
Multiplicity 3.6 3.3 3.7
I/sigma 6.2 14.7 1.2
Rmerge 0.155 0.036 2.348
CC half 0.984 0.998 0.393
Anomalous completeness 99.0 99.9 90.0
Anomalous multiplicity 1.9 1.9 1.9
Cell 116.016 36.307 125.163 90.0 93.844 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.