X-Ray Diffraction data from Rat Cerebellin-1, source of 5KWR structure

Data DOI: 10.15785/SBGRID/418 | ID: 418

Publication DOI: 10.1016/j.str.2016.11.004

5KWR Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Ozkan Laboratory, University of Chicago

Release Date: Jan. 17, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/418

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/418 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/418 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/418 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/418 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 418 ; shasum -c files.sha'

Storage requirements: 3.8G

Biological Sample:

Rat Cerebellin-1

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Beamline 23-ID-D, GM/CA, APS, Chicago, IL

Data Creation Date:

Nov. 30, 2015

Related Datasets:

None


Cite this Dataset

Cheng , S; Ozkan, E. 2017. "X-Ray Diffraction data for: Rat Cerebellin-1. PDB Code 5KWR", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/418.

Download Citation

Dataset Description

Rat Cerebellin-1, N-terminal domain degraded in the crystallization drops, resulting in the C1q-domain-only trimers.

Project Members

Name Additional Roles Affiliation While Working on the Project
Shouqiang Cheng Data CollectorUniversity of Chicago
Engin OzkanData Collector, Depositor, PIUniversity of Chicago

Reprocessing Instructions

Processed by XDS, all parameters are correctly saved in the image header, including the beam center values (ORGX= 1260.0, ORGY= 1284.0).

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03321

Sweep:

SWEEP1

Files:

/programs/datagrid/418/B12_7_#####.cbf

Images:

1 - 650

Beam:

(220.85, 216.72) -> (220.85, 216.84)

Distance:

380.0 -> 377.13

Spacegroup:

P 6

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.75 4.75 1.75
Low resolution limit 41.43 41.44 1.78
Completeness 96.5 99.8 75.1
Multiplicity 5.6 6.5 2.5
I/sigma 12.5 23.7 1.2
Rmerge 0.058 0.032 0.922
CC half 0.999 0.999 0.33
Anomalous completeness 84.5 96.4 41.2
Anomalous multiplicity 3.1 3.1 3.1
Cell 82.864 82.864 50.473 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03321

Sweep:

SWEEP1

Files:

/programs/datagrid/418/B12_7_#####.cbf

Images:

1 - 650

Beam:

(220.85, 216.72) -> (220.85, 216.91)

Distance:

380.0 -> 375.6

Spacegroup:

P 6

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.74 4.72 1.74
Low resolution limit 41.21 41.22 1.77
Completeness 96.6 99.7 80.4
Multiplicity 6.1 6.7 2.7
I/sigma 20.7 50.6 0.9
Rmerge 0.035 0.023 0.952
CC half 1.0 1.0 0.412
Anomalous completeness 86.9 96.8 49.7
Anomalous multiplicity 3.3 3.3 3.3
Cell 82.75 82.75 50.37 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03321

Sweep:

SWEEP1

Files:

/programs/datagrid/418/B12_7_#####.cbf

Images:

1 - 650

Beam:

(220.85, 216.72) -> (220.85, 216.9)

Distance:

380.0 -> 376.91

Spacegroup:

P 6

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.74 4.72 1.74
Low resolution limit 41.21 41.22 1.77
Completeness 96.9 99.9 80.6
Multiplicity 6.1 6.7 2.7
I/sigma 20.7 50.7 0.9
Rmerge 0.035 0.023 0.956
CC half 1.0 0.999 0.412
Anomalous completeness 87.1 96.8 49.9
Anomalous multiplicity 3.3 3.3 3.3
Cell 82.75 82.75 50.37 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03321

Sweep:

SWEEP1

Files:

/programs/datagrid/418/B12_7_#####.cbf

Images:

1 - 650

Beam:

(220.85, 216.72) -> (220.85, 216.89)

Distance:

380.0 -> 375.91

Spacegroup:

P 6

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.72 4.66 1.72
Low resolution limit 71.8 71.86 1.75
Completeness 95.3 99.8 61.9
Multiplicity 5.9 6.8 2.4
I/sigma 16.0 38.5 1.2
Rmerge 0.043 0.026 0.839
CC half 0.999 0.999 0.483
Anomalous completeness 84.0 97.4 32.5
Anomalous multiplicity 3.2 3.2 3.2
Cell 82.909 82.909 50.368 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.