X-Ray Diffraction data from Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+ & BeF3-, source of 5IUL structure

Data DOI: 10.15785/SBGRID/408 | ID: 408

Publication DOI: 10.7554/eLife.21422

5IUL Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Buschiazzo Laboratory, Institut Pasteur de Montevideo

Release Date: 13 Dec 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/408

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/408 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/408 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/408 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/408 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 408 ; shasum -c files.sha'

Storage requirements: 2.2G

Biological Sample:

Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+ & BeF3-

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Institut Pasteur de Montevideo Protein Crystallography Facility - RIGAKU MICROMAX-007 HF

Data Creation Date:

31 Oct 2015

Related Datasets:

None


Cite this Dataset

Trajtenberg, F; Buschiazzo, A. 2016. "X-Ray Diffraction data for: Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+ & BeF3-. PDB Code 5IUL", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/408.

Download Citation

Dataset Description

native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Alejandro BuschiazzoDepositorInstitut Pasteur de Montevideo / Institut Pasteur
Felipe TrajtenbergData CollectorInstitut Pasteur de Montevideo
Alejandro BuschiazzoPIInstitut Pasteur de Montevideo

Reprocessing Instructions

All images were eventually integrated, but indexing was achieved selecting frames 10-40 and 200-240 and using the 1000 strongest reflections.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.54179

Sweep:

SWEEP1

Files:

/programs/datagrid/408/20151031_KR_108_D4_x1_bef_###.mar2300

Images:

1 - 593

Beam:

(172.5, 172.5) -> (172.5, 172.61)

Distance:

300.0 -> 300.05

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.01 8.15 3.01
Low resolution limit 39.22 39.23 3.06
Completeness 96.1 88.7 81.8
Multiplicity 3.7 3.4 3.2
I/sigma 9.6 22.7 -0.4
Rmerge 0.079 0.041 -1.84
CC half 0.997 0.996 0.537
Anomalous completeness 74.8 69.3 49.7
Anomalous multiplicity 2.1 2.1 2.1
Cell 88.14 116.44 91.86 90.0 117.12 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.54179

Sweep:

SWEEP1

Files:

/programs/datagrid/408/20151031_KR_108_D4_x1_bef_###.mar2300

Images:

1 - 593

Beam:

(172.5, 172.5) -> (172.5, 172.6)

Distance:

300.0 -> 301.31

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.01 8.16 3.01
Low resolution limit 46.92 46.92 3.06
Completeness 96.1 88.9 83.7
Multiplicity 3.7 3.4 3.2
I/sigma 9.6 22.7 -0.4
Rmerge 0.079 0.041 -1.802
CC half 0.997 0.996 0.533
Anomalous completeness 74.9 69.6 51.5
Anomalous multiplicity 2.1 2.1 2.1
Cell 88.13 116.44 91.86 90.0 117.12 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.54179

Sweep:

SWEEP1

Files:

/programs/datagrid/408/20151031_KR_108_D4_x1_bef_###.mar2300

Images:

1 - 593

Beam:

(172.5, 172.5) -> (172.5, 172.59)

Distance:

300.0 -> 301.47

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.89 7.83 2.89
Low resolution limit 80.33 80.36 2.94
Completeness 90.6 98.5 60.9
Multiplicity 6.4 6.6 3.5
I/sigma 2.0 7.9 0.0
Rmerge 0.237 0.141 -0.991
CC half 0.891 0.99 0.289
Anomalous completeness 86.2 98.2 42.8
Anomalous multiplicity 3.5 3.5 3.5
Cell 94.186 116.974 153.857 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.