Data DOI: 10.15785/SBGRID/408 | ID: 408
Publication DOI: 10.7554/eLife.21422
5IUL Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Buschiazzo Laboratory, Institut Pasteur de Montevideo
Release Date: 13 Dec 2016
1. If this dataset is locally available, it should be accessable at /programs/datagrid/408
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/408 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/408 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/408 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/408 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 408 ; shasum -c files.sha'
Storage requirements: 2.2G
Biological Sample:
Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+ & BeF3-
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
Institut Pasteur de Montevideo Protein Crystallography Facility - RIGAKU MICROMAX-007 HF
Data Creation Date:
31 Oct 2015
Related Datasets:
None
Trajtenberg, F; Buschiazzo, A. 2016. "X-Ray Diffraction data for: Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+ & BeF3-. PDB Code 5IUL", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/408.
native dataset
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Alejandro Buschiazzo | Depositor | Institut Pasteur de Montevideo / Institut Pasteur |
| Felipe Trajtenberg | Data Collector | Institut Pasteur de Montevideo |
| Alejandro Buschiazzo | PI | Institut Pasteur de Montevideo |
All images were eventually integrated, but indexing was achieved selecting frames 10-40 and 200-240 and using the 1000 strongest reflections.
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 1.54179
Sweep:
SWEEP1
Files:
/programs/datagrid/408/20151031_KR_108_D4_x1_bef_###.mar2300
Images:
1 - 593
Beam:
(172.5, 172.5) -> (172.5, 172.61)
Distance:
300.0 -> 300.05
Spacegroup:
P 1 21 1
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 3.01 | 8.15 | 3.01 |
| Low resolution limit | 39.22 | 39.23 | 3.06 |
| Completeness | 96.1 | 88.7 | 81.8 |
| Multiplicity | 3.7 | 3.4 | 3.2 |
| I/sigma | 9.6 | 22.7 | -0.4 |
| Rmerge | 0.079 | 0.041 | -1.84 |
| CC half | 0.997 | 0.996 | 0.537 |
| Anomalous completeness | 74.8 | 69.3 | 49.7 |
| Anomalous multiplicity | 2.1 | 2.1 | 2.1 |
| Cell | 88.14 | 116.44 | 91.86 | 90.0 | 117.12 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 1.54179
Sweep:
SWEEP1
Files:
/programs/datagrid/408/20151031_KR_108_D4_x1_bef_###.mar2300
Images:
1 - 593
Beam:
(172.5, 172.5) -> (172.5, 172.6)
Distance:
300.0 -> 301.31
Spacegroup:
P 1 21 1
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 3.01 | 8.16 | 3.01 |
| Low resolution limit | 46.92 | 46.92 | 3.06 |
| Completeness | 96.1 | 88.9 | 83.7 |
| Multiplicity | 3.7 | 3.4 | 3.2 |
| I/sigma | 9.6 | 22.7 | -0.4 |
| Rmerge | 0.079 | 0.041 | -1.802 |
| CC half | 0.997 | 0.996 | 0.533 |
| Anomalous completeness | 74.9 | 69.6 | 51.5 |
| Anomalous multiplicity | 2.1 | 2.1 | 2.1 |
| Cell | 88.13 | 116.44 | 91.86 | 90.0 | 117.12 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 1.54179
Sweep:
SWEEP1
Files:
/programs/datagrid/408/20151031_KR_108_D4_x1_bef_###.mar2300
Images:
1 - 593
Beam:
(172.5, 172.5) -> (172.5, 172.59)
Distance:
300.0 -> 301.47
Spacegroup:
P 21 21 21
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.89 | 7.83 | 2.89 |
| Low resolution limit | 80.33 | 80.36 | 2.94 |
| Completeness | 90.6 | 98.5 | 60.9 |
| Multiplicity | 6.4 | 6.6 | 3.5 |
| I/sigma | 2.0 | 7.9 | 0.0 |
| Rmerge | 0.237 | 0.141 | -0.991 |
| CC half | 0.891 | 0.99 | 0.289 |
| Anomalous completeness | 86.2 | 98.2 | 42.8 |
| Anomalous multiplicity | 3.5 | 3.5 | 3.5 |
| Cell | 94.186 | 116.974 | 153.857 | 90.0 | 90.0 | 90.0 |
|---|
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
