X-Ray Diffraction data from Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+, source of 5IUK structure

Data DOI: 10.15785/SBGRID/401 | ID: 401

Publication DOI: 10.7554/eLife.21422

5IUK Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Buschiazzo Laboratory, Institut Pasteur de Montevideo

Release Date: Dec. 13, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/401

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/401 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/401 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/401 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/401 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 401 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SYNCHROTRON SOLEIL BEAMLINE PROXIMA 1

Data Creation Date:

Feb. 14, 2016

Related Datasets:

None


Cite this Dataset

Trajtenberg, F; Buschiazzo, A. 2016. "X-Ray Diffraction data for: Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+. PDB Code 5IUK", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/401.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Alejandro BuschiazzoDepositorInstitut Pasteur de Montevideo / Institut Pasteur
Felipe TrajtenbergData Collectorinstitut Pasteur de Montevideo
Alejandro BuschiazzoPIInstitut Pasteur de Montevideo

Reprocessing Instructions

These are two overlapping sets of images, with root file names IP7_4_1 and IP7_4_2 (1000 frames each). Our best processing strategy to get one complete integrated/scaled dataset (the one effectively used to determine and refine the published structure), used the first 800 frames from set IP7_4_1, merged with frames 1-530 and 650-910 from set IP7_4_2.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97857

Sweep:

SWEEP2

Files:

/programs/datagrid/401/IP7_4_2_####.cbf

Images:

1 - 1000

Beam:

(224.96, 203.09) -> (224.96, 203.12)

Distance:

393.4 -> 392.16

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.9 7.86 2.9
Low resolution limit 43.6 43.6 2.95
Completeness 99.5 96.5 99.8
Multiplicity 5.0 6.6 3.8
I/sigma 11.6 41.8 1.1
Rmerge 0.063 0.031 1.212
CC half 0.998 0.998 0.537
Anomalous completeness 94.3 90.5 94.2
Anomalous multiplicity 2.6 2.6 2.6
Cell 87.93 115.59 91.6 90.0 116.71 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97857

Sweep:

SWEEP2

Files:

/programs/datagrid/401/IP7_4_2_####.cbf

Images:

1 - 1000

Beam:

(224.96, 203.09) -> (224.96, 203.14)

Distance:

393.4 -> 393.56

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.87 7.79 2.87
Low resolution limit 78.56 78.59 2.92
Completeness 99.5 96.7 99.6
Multiplicity 5.0 6.6 3.6
I/sigma 11.3 41.2 1.0
Rmerge 0.064 0.031 1.226
CC half 0.998 0.998 0.501
Anomalous completeness 94.3 90.8 89.5
Anomalous multiplicity 2.6 2.6 2.6
Cell 87.94 115.6 91.61 90.0 116.71 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97857

Sweep:

SWEEP2

Files:

/programs/datagrid/401/IP7_4_2_####.cbf

Images:

1 - 1000

Beam:

(224.96, 203.09) -> (224.96, 203.14)

Distance:

393.4 -> 392.9

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.67 9.95 3.67
Low resolution limit 115.57 115.63 3.73
Completeness 98.9 99.7 96.2
Multiplicity 2.9 3.8 1.8
I/sigma 7.5 11.5 3.7
Rmerge 0.066 0.052 0.146
CC half 0.991 0.996 0.7
Anomalous completeness 85.5 94.1 64.7
Anomalous multiplicity 1.5 1.5 1.5
Cell 87.951 91.596 115.571 89.942 89.957 63.282

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.