Data DOI: 10.15785/SBGRID/401 | ID: 401
Publication DOI: 10.7554/eLife.21422
5IUK Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Buschiazzo Laboratory, Institut Pasteur de Montevideo
Release Date: 13 Dec 2016
1. If this dataset is locally available, it should be accessable at /programs/datagrid/401
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/401 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/401 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/401 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/401 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 401 ; shasum -c files.sha'
Storage requirements: 12G
Biological Sample:
Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
SYNCHROTRON SOLEIL BEAMLINE PROXIMA 1
Data Creation Date:
14 Feb 2016
Related Datasets:
None
Trajtenberg, F; Buschiazzo, A. 2016. "X-Ray Diffraction data for: Complex DesKC:DesR-REC (B. subtilis) - phosphotransferase state, high Mg2+. PDB Code 5IUK", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/401.
Native dataset
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Alejandro Buschiazzo | Depositor | Institut Pasteur de Montevideo / Institut Pasteur |
| Felipe Trajtenberg | Data Collector | institut Pasteur de Montevideo |
| Alejandro Buschiazzo | PI | Institut Pasteur de Montevideo |
These are two overlapping sets of images, with root file names IP7_4_1 and IP7_4_2 (1000 frames each). Our best processing strategy to get one complete integrated/scaled dataset (the one effectively used to determine and refine the published structure), used the first 800 frames from set IP7_4_1, merged with frames 1-530 and 650-910 from set IP7_4_2.
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97857
Sweep:
SWEEP2
Files:
/programs/datagrid/401/IP7_4_2_####.cbf
Images:
1 - 1000
Beam:
(224.96, 203.09) -> (224.96, 203.13)
Distance:
393.4 -> 391.17
Spacegroup:
P 1 21 1
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.76 | 7.48 | 2.76 |
| Low resolution limit | 43.6 | 43.6 | 2.81 |
| Completeness | 99.7 | 97.5 | 99.9 |
| Multiplicity | 6.9 | 6.6 | 3.8 |
| I/sigma | 10.2 | 37.7 | 0.5 |
| Rmerge | 0.094 | 0.034 | 2.168 |
| CC half | 0.998 | 0.998 | 0.328 |
| Anomalous completeness | 97.6 | 92.4 | 95.2 |
| Anomalous multiplicity | 3.5 | 3.5 | 3.5 |
| Cell | 87.93 | 115.59 | 91.6 | 90.0 | 116.71 | 90.0 |
|---|
Version:
version unreported
Reprocessing failed.
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97857
Sweep:
SWEEP2
Files:
/programs/datagrid/401/IP7_4_2_####.cbf
Images:
1 - 1000
Beam:
(224.96, 203.09) -> (224.96, 203.14)
Distance:
393.4 -> 392.89
Spacegroup:
P 1 21 1
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.64 | 7.18 | 2.64 |
| Low resolution limit | 66.77 | 66.79 | 2.69 |
| Completeness | 99.8 | 100.0 | 94.7 |
| Multiplicity | 7.6 | 7.3 | 7.6 |
| I/sigma | 5.9 | 37.6 | 0.1 |
| Rmerge | 0.15 | 0.056 | 4.973 |
| CC half | 0.995 | 0.993 | 0.204 |
| Anomalous completeness | 99.7 | 100.0 | 94.8 |
| Anomalous multiplicity | 3.9 | 3.9 | 3.9 |
| Cell | 87.904 | 115.414 | 91.554 | 90.0 | 116.618 | 90.0 |
|---|
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
