Data DOI: 10.15785/SBGRID/365 | ID: 365
Publication DOI: 10.7554/eLife.18740
5KJ7 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Brunger Laboratory, Stanford University
Release Date: 28 Oct 2016
1. If this dataset is locally available, it should be accessable at /programs/datagrid/365
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/365 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/365 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/365 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/365 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 365 ; shasum -c files.sha'
Storage requirements: 25G
Biological Sample:
Synaptotagmin-1 / SNARE complex
Dataset Type:
XFEL Diffraction
Subject Composition:
Protein
Collection Facility:
XPP / LCLS
Data Creation Date:
20 May 2014
Related Datasets:
None
Lyubimov, AY; Uervirojnangkoorn, M; Zhao, M; Zhou, Q; Brunger, AT. 2016. "XFEL Diffraction data for: Synaptotagmin-1 / SNARE complex. PDB Code 5KJ7", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/365.
The XFEL data were collected at the X-ray Pump Probe (XPP) endstation of the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, using a goniometer-based fixed target sample delivery station and an automatic sample loading system. We used a 30-micron XFEL beam with a pulse duration of 40 fs in SASE mode. A total of 148 crystals were screened, yielding 789 images with usable diffraction.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Artem Y Lyubimov | Data Collector, Depositor | HHMI / Stanford University |
| Axel T Brunger | HHMI / Stanford University | |
| Monarin Uervirojnangkoorn | Data Collector | Stanford University |
| Minglei Zhao | Data Collector | Stanford University |
| Qiangjun Zhou | Data Collector | Stanford University |
| Axel Brunger | PI | Stanford University |
Please use the IOTA and PRIME programs included in Phenix dev-2645 or later. Detailed instructions on loading that version of Phenix and using the data processing software are supplied in README.txt, which is included in the processing bundle.
Processing Bundle (sha1: f79ad39ff15e1c6b8f4dca9bcc5d53e3a5e4e3e7)
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
