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XFEL Diffraction data from Synaptotagmin-1 / SNARE complex, source of 5KJ7 structure

Data DOI: 10.15785/SBGRID/365 | ID: 365

Publication DOI: 10.7554/eLife.18740

5KJ7 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Brunger Laboratory, Stanford University

Release Date: Oct. 28, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/365

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/365 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/365 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/365 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/365 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 365 ; shasum -c files.sha'

Storage requirements: 25G

Biological Sample:

Synaptotagmin-1 / SNARE complex

Dataset Type:

XFEL Diffraction

Subject Composition:

Protein

Collection Facility:

XPP / LCLS

Data Creation Date:

May 20, 2014

Related Datasets:

None


Cite this Dataset

Lyubimov, AY; Uervirojnangkoorn, M; Zhao, M; Zhou, Q; Brunger, AT. 2016. "XFEL Diffraction data for: Synaptotagmin-1 / SNARE complex. PDB Code 5KJ7", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/365.

Download Citation

Dataset Description

The XFEL data were collected at the X-ray Pump Probe (XPP) endstation of the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, using a goniometer-based fixed target sample delivery station and an automatic sample loading system. We used a 30-micron XFEL beam with a pulse duration of 40 fs in SASE mode. A total of 148 crystals were screened, yielding 789 images with usable diffraction.

Project Members

Name Additional Roles Affiliation While Working on the Project
Artem Y LyubimovData Collector, DepositorHHMI / Stanford University
Axel T BrungerHHMI / Stanford University
Monarin UervirojnangkoornData CollectorStanford University
Minglei ZhaoData CollectorStanford University
Qiangjun ZhouData CollectorStanford University
Axel BrungerPIStanford University

Reprocessing Instructions

Please use the IOTA and PRIME programs included in Phenix dev-2645 or later. Detailed instructions on loading that version of Phenix and using the data processing software are supplied in README.txt, which is included in the processing bundle.

Processing Bundle (sha1: f79ad39ff15e1c6b8f4dca9bcc5d53e3a5e4e3e7)


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.