X-Ray Diffraction data from Human Protocadherin-15 EC3-5 D414A Variant, source of 5T4N structure

Data DOI: 10.15785/SBGRID/363 | ID: 363

5T4N Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Gaudet Laboratory, Harvard University

Release Date: Feb. 14, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/363

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/363 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/363 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/363 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/363 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 363 ; shasum -c files.sha'

Storage requirements: 2.0G

Biological Sample:

Human Protocadherin-15 EC3-5 D414A Variant

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

24-ID-E, NE-CAT, APS, Chicago, IL

Data Creation Date:

Nov. 8, 2013

Related Datasets:

None


Cite this Dataset

Powers, RE; Sotomayor, M; Gaudet, R. 2017. "X-Ray Diffraction data for: Human Protocadherin-15 EC3-5 D414A Variant. PDB Code 5T4N", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/363.

Download Citation

Dataset Description

Native dataset used to determine the structure of Human Protocadherin-15 EC3-5 D414A Variant using molecular replacement.

Project Members

Name Additional Roles Affiliation While Working on the Project
Robert E PowersData Collector, DepositorBiophysics Graduate Program, Harvard University, Boston MA 02115
Rachelle GaudetDepartment of Molecular and Cellular Biology, Harvard University, Cambridge MA 02138
Marcos SotomayorDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus OH 43210
Rachelle GaudetPIHarvard University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP1

Files:

/programs/datagrid/363/RG001_05_02_2_###.img

Images:

1 - 110

Beam:

(152.93, 158.74) -> (152.93, 158.86)

Distance:

400.0 -> 400.08

Spacegroup:

P 61

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.84 7.7 2.84
Low resolution limit 52.49 52.5 2.89
Completeness 99.8 99.0 100.0
Multiplicity 7.0 7.0 7.0
I/sigma 11.0 31.1 1.2
Rmerge 0.149 0.035 1.608
CC half 0.996 0.999 0.386
Anomalous completeness 94.7 97.1 93.0
Anomalous multiplicity 3.6 3.6 3.6
Cell 105.2 105.2 192.638 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP1

Files:

/programs/datagrid/363/RG001_05_02_2_###.img

Images:

1 - 110

Beam:

(152.93, 158.74) -> (152.93, 158.81)

Distance:

400.0 -> 400.1

Spacegroup:

P 6

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.88 7.81 2.88
Low resolution limit 50.76 50.77 2.93
Completeness 99.6 97.6 99.9
Multiplicity 7.1 7.0 7.1
I/sigma 12.9 42.3 1.1
Rmerge 0.118 0.029 1.697
CC half 0.998 0.999 0.37
Anomalous completeness 94.5 95.4 93.4
Anomalous multiplicity 3.7 3.7 3.7
Cell 105.24 105.24 192.6 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP1

Files:

/programs/datagrid/363/RG001_05_02_2_###.img

Images:

1 - 110

Beam:

(152.93, 158.74) -> (152.93, 158.81)

Distance:

400.0 -> 400.02

Spacegroup:

P 6

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.87 7.78 2.87
Low resolution limit 50.76 50.77 2.92
Completeness 99.6 97.6 99.9
Multiplicity 7.1 7.0 7.1
I/sigma 12.8 42.2 1.1
Rmerge 0.12 0.029 1.716
CC half 0.998 0.999 0.341
Anomalous completeness 94.5 95.5 93.6
Anomalous multiplicity 3.7 3.7 3.7
Cell 105.24 105.24 192.6 90.0 90.0 120.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.