X-Ray Diffraction data from Human Dpf3 double-PHD domain bound to histone H3 tail peptide with monomethylated K4/acetylated K14, source of 5SZC structure

Data DOI: 10.15785/SBGRID/359 | ID: 359

5SZC Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Corbett Laboratory, University of California, San Diego

Release Date: 5 Sep 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/359

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/359 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/359 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/359 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/359 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 359 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

Human Dpf3 double-PHD domain bound to histone H3 tail peptide with monomethylated K4/acetylated K14

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL Beamline 9-2

Data Creation Date:

14 Jun 2016

Related Datasets:

None


Cite this Dataset

Local, A; Ren, B; Shiau, AK; Singh, N; Corbett, KD. 2017. "X-Ray Diffraction data for: Human Dpf3 double-PHD domain bound to histone H3 tail peptide with monomethylated K4/acetylated K14. PDB Code 5SZC", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/359.

Download Citation

Dataset Description

Native data for PDB entry 5SZC

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettDepositorUniversity of California, San Diego and Ludwig Institute for Cancer Research
Andrea LocalUniversity of California, San Diego and Ludwig Institute for Cancer Research
Bing RenUniversity of California, San Diego and Ludwig Institute for Cancer Research
Andrew K ShiauLudwig Institute for Cancer Research
Namit SinghData CollectorUniversity of California, San Diego and Ludwig Institute for Cancer Research
Kevin CorbettPIUniversity of California, San Diego

Reprocessing Instructions

Process to 1.2 A with XDS, Space group P4(3)2(1)2

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/359/DPF3_2349_1_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.4)

Distance:

200.0 -> 198.7

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.03 2.8 1.03
Low resolution limit 28.02 28.03 1.05
Completeness 85.6 100.0 15.3
Multiplicity 10.5 11.5 2.1
I/sigma 30.9 80.7 0.5
Rmerge 0.03 0.021 1.295
CC half 1.0 1.0 0.245
Anomalous completeness 82.6 100.0 6.3
Anomalous multiplicity 5.7 5.7 5.7
Cell 39.62 39.62 148.58 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/359/DPF3_2349_1_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.42)

Distance:

200.0 -> 199.12

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.04 2.82 1.04
Low resolution limit 38.28 38.31 1.06
Completeness 87.5 100.0 25.2
Multiplicity 10.5 11.6 2.8
I/sigma 30.9 80.7 0.7
Rmerge 0.03 0.021 1.1
CC half 1.0 1.0 0.264
Anomalous completeness 84.7 100.0 15.0
Anomalous multiplicity 5.7 5.7 5.7
Cell 39.62 39.62 148.58 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.