Data DOI: 10.15785/SBGRID/356 | ID: 356
Iverson Laboratory, Vanderbilt University
Release Date: 20 Nov 2018
1. If this dataset is locally available, it should be accessable at /programs/datagrid/356
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/356 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/356 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/356 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/356 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 356 ; shasum -c files.sha'
Storage requirements: 8.6G
Biological Sample:
Methyltransferase domain of EvdMO1 crystallized in the presence of 2 mM SAH + 10 mM fucose
Dataset Type:
X-Ray Diffraction
Subject Composition:
Ligand, Protein
Collection Facility:
APS LS-CAT 21-ID-F
Data Creation Date:
3 Jun 2016
Related Datasets:
None
McCulloch, KM; Iverson, TM. 2018. "X-Ray Diffraction data for: Methyltransferase domain of EvdMO1 crystallized in the presence of 2 mM SAH + 10 mM fucose.", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/356.
X-ray diffraction data for the methyltransferase domain of EvdMO1 (EvdMdO1) to 1.10 A resolution. Data were collected from a sample co-crystallized in the presence of 2 mM SAH and 10 mM fucose. No density for the fucose is observed, but an active site loop becomes disordered in the presence of fucose.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Kathryn M McCulloch | Depositor | Vanderbilt University |
| Tina M Iverson | Data Collector, PI | Vanderbilt University |
none
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97872
Sweep:
SWEEP1
Files:
/programs/datagrid/356/10mMfuc-12h.###
Images:
1 - 275
Beam:
(149.84, 149.61) -> (149.84, 149.57)
Distance:
115.0 -> 115.02
Spacegroup:
P 1
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.85 | 5.02 | 1.85 |
| Low resolution limit | 37.21 | 37.22 | 1.88 |
| Completeness | 95.7 | 92.6 | 95.9 |
| Multiplicity | 2.3 | 2.2 | 2.4 |
| I/sigma | 0.7 | 0.6 | 0.6 |
| Rmerge | 0.874 | 1.236 | 0.913 |
| CC half | 0.058 | 0.006 | 0.214 |
| Anomalous completeness | 85.9 | 80.4 | 88.9 |
| Anomalous multiplicity | 1.2 | 1.2 | 1.2 |
| Cell | 51.937 | 58.485 | 81.976 | 90.0 | 90.0 | 99.093 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97872
Sweep:
SWEEP1
Files:
/programs/datagrid/356/10mMfuc-12h.###
Images:
1 - 275
Beam:
(149.84, 149.61) -> (149.84, 150.36)
Distance:
115.0 -> 115.27
Spacegroup:
P 1 2 1
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 3.07 | 8.3 | 3.07 |
| Low resolution limit | 34.14 | 34.15 | 3.12 |
| Completeness | 99.9 | 100.0 | 100.0 |
| Multiplicity | 3.5 | 3.5 | 3.7 |
| I/sigma | 0.9 | 0.3 | 1.0 |
| Rmerge | 0.601 | 0.96 | 0.608 |
| CC half | 0.166 | 0.034 | 0.083 |
| Anomalous completeness | 93.2 | 89.9 | 95.9 |
| Anomalous multiplicity | 1.9 | 1.9 | 1.9 |
| Cell | 49.84 | 41.02 | 57.9 | 90.0 | 100.62 | 90.0 |
|---|
Version:
version unreported
Reprocessing failed.
Version:
version unreported
Reprocessing failed.
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.