X-Ray Diffraction data from Methyltransferase domain of EvdMO1 crystallized in the presence of 2 mM SAH + 10 mM fucose, source of structure

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Data DOI: 10.15785/SBGRID/356 | ID: 356

Iverson Laboratory, Vanderbilt University

Release Date: 20 Nov 2018

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/356

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/356 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/356 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/356 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/356 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 356 ; shasum -c files.sha'

Storage requirements: 8.6G

Biological Sample:

Methyltransferase domain of EvdMO1 crystallized in the presence of 2 mM SAH + 10 mM fucose

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

APS LS-CAT 21-ID-F

Data Creation Date:

3 Jun 2016

Related Datasets:

None


Cite this Dataset

McCulloch, KM; Iverson, TM. 2018. "X-Ray Diffraction data for: Methyltransferase domain of EvdMO1 crystallized in the presence of 2 mM SAH + 10 mM fucose.", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/356.

Download Citation

Dataset Description

X-ray diffraction data for the methyltransferase domain of EvdMO1 (EvdMdO1) to 1.10 A resolution. Data were collected from a sample co-crystallized in the presence of 2 mM SAH and 10 mM fucose. No density for the fucose is observed, but an active site loop becomes disordered in the presence of fucose.

Project Members

Name Additional Roles Affiliation While Working on the Project
Kathryn M McCullochDepositorVanderbilt University
Tina M IversonData Collector, PIVanderbilt University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/356/10mMfuc-12h.###

Images:

1 - 275

Beam:

(149.84, 149.61) -> (149.84, 149.57)

Distance:

115.0 -> 115.02

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.85 5.02 1.85
Low resolution limit 37.21 37.22 1.88
Completeness 95.7 92.6 95.9
Multiplicity 2.3 2.2 2.4
I/sigma 0.7 0.6 0.6
Rmerge 0.874 1.236 0.913
CC half 0.058 0.006 0.214
Anomalous completeness 85.9 80.4 88.9
Anomalous multiplicity 1.2 1.2 1.2
Cell 51.937 58.485 81.976 90.0 90.0 99.093

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/356/10mMfuc-12h.###

Images:

1 - 275

Beam:

(149.84, 149.61) -> (149.84, 150.36)

Distance:

115.0 -> 115.27

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.07 8.3 3.07
Low resolution limit 34.14 34.15 3.12
Completeness 99.9 100.0 100.0
Multiplicity 3.5 3.5 3.7
I/sigma 0.9 0.3 1.0
Rmerge 0.601 0.96 0.608
CC half 0.166 0.034 0.083
Anomalous completeness 93.2 89.9 95.9
Anomalous multiplicity 1.9 1.9 1.9
Cell 49.84 41.02 57.9 90.0 100.62 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.