X-Ray Diffraction data from nanobody-bound viral GPCR bound to human chemokine CX3CL1, source of 4XT1 structure

Data DOI: 10.15785/SBGRID/339 | ID: 339

Publication DOI: 10.1126/science.aaa5026

4XT1 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: Aug. 2, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/339

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/339 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/339 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/339 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/339 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 339 ; shasum -c files.sha'

Storage requirements: 15G

Biological Sample:

nanobody-bound viral GPCR bound to human chemokine CX3CL1

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

23 ID-D, GM/CA, APS, Chicago, IL

Data Creation Date:

Oct. 13, 2014

Related Datasets:

None


Cite this Dataset

Jude, KM; Burg, JS; Garcia, K. 2016. "X-Ray Diffraction data for: nanobody-bound viral GPCR bound to human chemokine CX3CL1. PDB Code 4XT1", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/339.

Download Citation

Dataset Description

native datasets

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorStanford University
John S BurgData CollectorStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

datasets D8-27-42-19_1 (frames 1-30), D1-47-48-24_1 (frames 1-30), D1-73-43_1 (frames 1-72), D1-81-28-27_1 (frames 1-10), and D1-30-24-8_1 (frames 1-28) were processed and merged in HKL2000 to 2.75 Å resolution

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0332

Sweep:

SWEEP4

Files:

/programs/datagrid/339/D8-27-42-19_1.####

Images:

1 - 90

Beam:

(150.51, 150.0) -> (150.51, 150.02)

Distance:

350.0 -> 349.71

Spacegroup:

I 4

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.9 7.87 2.9
Low resolution limit 57.24 57.25 2.95
Completeness 99.8 99.6 97.6
Multiplicity 16.7 19.3 4.3
I/sigma 12.4 41.5 1.0
Rmerge 0.215 0.065 1.336
CC half 0.997 0.999 0.392
Anomalous completeness 98.8 99.7 80.7
Anomalous multiplicity 8.5 8.5 8.5
Cell 80.95 80.95 230.64 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0332

Sweep:

SWEEP4

Files:

/programs/datagrid/339/D8-27-42-19_1.####

Images:

1 - 90

Beam:

(150.51, 150.0) -> (150.51, 150.02)

Distance:

350.0 -> 350.35

Spacegroup:

I 4

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.92 7.92 2.92
Low resolution limit 57.24 57.25 2.97
Completeness 99.9 99.6 99.1
Multiplicity 16.9 19.3 7.2
I/sigma 12.6 41.7 1.3
Rmerge 0.215 0.066 1.414
CC half 0.993 0.993 0.505
Anomalous completeness 99.5 99.7 92.7
Anomalous multiplicity 8.6 8.6 8.6
Cell 80.95 80.95 230.63 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.