X-Ray Diffraction data from N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18), source of 2FW6 structure

Data DOI: 10.15785/SBGRID/292 | ID: 292

Publication DOI: 10.1021/bi060465n

2FW6 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Kappock Laboratory, Purdue University

Release Date: May 31, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/292

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/292 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/292 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/292 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/292 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 292 ; shasum -c files.sha'

Storage requirements: 2.0G

Biological Sample:

N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

RIGAKU RAXIS IV

Data Creation Date:

July 27, 2005

Related Datasets:

None


Cite this Dataset

Starks, CM.; Kappock, T. 2016. "X-Ray Diffraction data for: N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18). PDB Code 2FW6", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/292.

Download Citation

Dataset Description

Structure of PurE (N5-carboxyaminoimidazole ribonucleotide mutase) mutant H59N from the acidophilic bacterium Acetobacter aceti, at pH 5.4

Project Members

Name Additional Roles Affiliation While Working on the Project
T. Joseph KappockDepositorWashington University in St Louis
Courtney M. StarksData CollectorWashington University in St Louis
T. Joseph KappockPIPurdue University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.5418

Sweep:

SWEEP1

Files:

/programs/datagrid/292/h59n###.osc

Images:

1 - 119

Beam:

(140.33, 144.58) -> (140.33, 150.41)

Distance:

-150.0 -> 150.0

Spacegroup:

I 4 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.68 4.57 1.68
Low resolution limit 49.36 49.38 1.71
Completeness 83.6 92.9 10.5
Multiplicity 7.9 9.6 1.3
I/sigma 20.2 60.7 0.9
Rmerge 0.065 0.024 0.752
CC half 0.999 0.999 0.335
Anomalous completeness 78.6 94.3 1.7
Anomalous multiplicity 4.3 4.3 4.3
Cell 98.717 98.717 165.726 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.5418

Sweep:

SWEEP1

Files:

/programs/datagrid/292/h59n###.osc

Images:

1 - 119

Beam:

(140.33, 144.58) -> (140.33, 144.62)

Distance:

-150.0 -> -150.78

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.9 7.86 2.9
Low resolution limit 90.78 90.83 2.95
Completeness 75.7 68.1 76.2
Multiplicity 1.5 1.7 1.4
I/sigma 0.3 0.4 0.2
Rmerge 0.994 0.0 1.027
CC half -0.003 0.022 -0.045
Anomalous completeness 37.6 47.1 33.1
Anomalous multiplicity 1.0 1.0 1.0
Cell 95.664 100.119 101.021 65.287 80.225 89.02

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.