Micro-Electron Diffraction data from Thermolysin, source of 5k7t structure

Data DOI: 10.15785/SBGRID/290 | ID: 290

Publication DOI: 10.1038/nmeth.4178

5k7t Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

EMDB 8222: RCSB PDBe

Gonen Laboratory, University of California, Los Angeles

Release Date: March 31, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/290

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/290 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/290 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/290 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/290 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 290 ; shasum -c files.sha'

Storage requirements: 5.7G

Biological Sample:

Thermolysin

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Protein

Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

April 10, 2015

Related Datasets:

None


Cite this Dataset

Hattne, J; de la Cruz, J; Gonen, T. 2017. "Micro-Electron Diffraction data for: Thermolysin. PDB Code 5k7t", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/290.

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Dataset Description

Micro-electron diffraction data from thermolysin

Project Members

Name Additional Roles Affiliation While Working on the Project
Johan HattneDepositorHHMI Janelia Research Campus
Jason de la CruzData CollectorHHMI Janelia Research Campus
Tamir GonenPIUniversity of California, Los Angeles

Reprocessing Instructions

Convert corrected images to SMV: $ img2px -d 2640 -s 9.0 -o thermolysin_041015_01/thermo_###.img movie1-5m4s-2+_sq10_05_00?.tvips $ img2px -d 3240 -s 9.0 -o thermolysin_041615_09/thermo_###.img movie1-75m4s-2+_sq01_02_00?.tvips $ img2px -d 3240 -s 9.0 -o thermolysin_041615_19/thermo_###.img movie1-75m4s-2+_sq12_02_00?.tvips $ img2px -d 2640 -s 5.1 -o 20160314_sq_01_01/thermo_###.img 1-5m_4s_sq01_01_000.tvips MOSFLM processing parameters: DATASET ADC offset GAIN Direction of spindle rotation movie1-5m4s-2+_sq10_05 40 4 Forward movie1-75m4s-2+_sq01_02 10 5 Forward movie1-75m4s-2+_sq12_02 40 5 Forward 1-5m_4s_sq01_01 14 5 Forward


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.