Micro-Electron Diffraction data from Proteinase K, source of 5k7s structure

Data DOI: 10.15785/SBGRID/289 | ID: 289

Publication DOI: 10.1038/nmeth.4178

5k7s Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

EMDB 8221: RCSB PDBe

Gonen Laboratory, University of California, Los Angeles

Release Date: 31 Mar 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/289

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/289 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/289 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/289 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/289 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 289 ; shasum -c files.sha'

Storage requirements: 2.4G

Biological Sample:

Proteinase K

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Protein

Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

7 Mar 2016

Related Datasets:

None


Cite this Dataset

Hattne, J; de la Cruz, J; Gonen, T. 2017. "Micro-Electron Diffraction data for: Proteinase K. PDB Code 5k7s", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/289.

Download Citation

Dataset Description

Micro-electron diffraction data from proteinase K

Project Members

Name Additional Roles Affiliation While Working on the Project
Johan HattneDepositorHHMI Janelia Research Campus
Jason de la CruzData CollectorHHMI Janelia Research Campus
Tamir GonenPIUniversity of California, Los Angeles

Reprocessing Instructions

Convert corrected images to SMV: $ img2px -d 2200 -s 4.9 -o movie_01_01/prot_###.img p01_cl1200mm5sp01mv01_000.tvips $ img2px -d 2200 -s 5.8 -o movie_05_01/prot_###.img p05_cl1200mm5sp05mv01_000.tvips $ img2px -d 2200 -s 10.0 -o movie_06_01/prot_###.img p06_cl1200mm5sp05mv01_000.tvips $ img2px -d 2200 -s 11.0 -o movie_08_01/prot_###.img p08_cl1200mm5sp05mv01_000.tvips $ img2px -d 2200 -s 12.0 -o movie_09_01/prot_###.img p09_cl1200mm5sp05mv01_000.tvips $ img2px -d 2200 -s 6.0 -o movie_10_01/prot_###.img p10_cl1200mm5sp05mv01_000.tvips MOSFLM processing parameters: DATASET ADC offset GAIN Direction of spindle rotation p01_cl1200mm5sp01mv01 14 5 Forward p05_cl1200mm5sp05mv01 13 5 Forward p06_cl1200mm5sp05mv01 11 5 Forward p08_cl1200mm5sp05mv01 7 5 Forward p09_cl1200mm5sp05mv01 7 5 Forward p10_cl1200mm5sp05mv01 12 5 Forward


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.