Micro-Electron Diffraction data from Trypsin, source of 5k7r structure

Data DOI: 10.15785/SBGRID/288 | ID: 288

Publication DOI: 10.1038/nmeth.4178

5k7r Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

EMDB 8220: RCSB PDBe

Gonen Laboratory, University of California, Los Angeles

Release Date: March 31, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/288

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/288 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/288 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/288 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/288 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 288 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

Trypsin

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Protein

Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

March 10, 2016

Related Datasets:

None


Cite this Dataset

Hattne, J; de la Cruz, J; Gonen, T. 2017. "Micro-Electron Diffraction data for: Trypsin. PDB Code 5k7r", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/288.

Download Citation

Dataset Description

Micro-electron diffraction data from trypsin

Project Members

Name Additional Roles Affiliation While Working on the Project
Johan HattneDepositorHHMI Janelia Research Campus
Jason de la CruzData CollectorHHMI Janelia Research Campus
Tamir GonenPIUniversity of California, Los Angeles

Reprocessing Instructions

Convert corrected images to SMV: $ img2px -d 2640 -s 6.0 -o 20160310_sq_10_02/tryp_###.img 1-5m_4s_sq10_02_000.tvips $ img2px -d 3080 -s 5.0 -o 20160310_sq_05_01/tryp_###.img 1-75m_4s_sq05_01_000.tvips $ img2px -d 3080 -s 5.0 -o 20160310_sq_09_02/tryp_###.img 1-75m_4s_sq09_02_000.tvips $ img2px -d 2640 -s 4.8 -o 20160318_sq_01_01/tryp_###.img 1-5m_4s_sq01_01_000.tvips $ img2px -d 2640 -s 5.0 -o 20160318_sq_04_01/tryp_###.img 1-5m_4s_sq04_01_000.tvips $ img2px -d 2640 -s 5.0 -o 20160318_sq_04_02/tryp_###.img 1-5m_4s_sq04_02_000.tvips $ img2px -d 2640 -s 4.6 -o 20160318_sq_11_01/tryp_###.img 1-5m_4s_sq11_01_000.tvips $ img2px -d 2640 -s 4.4 -o 20160318_sq_11_02/tryp_###.img 1-5m_4s_sq11_02_000.tvips $ img2px -d 2640 -s 5.2 -o 20160318_sq_15_01/tryp_###.img 1-5m_4s_sq15_01_000.tvips $ img2px -d 2640 -s 5.8 -o 20160316_sq_07_01/tryp_###.img 1-5m_4s_sq07_01_000.tvips MOSFLM processing parameters: DATASET ADC offset GAIN Direction of spindle rotation 1-5m_4s_sq10_02 13 5 Forward 1-75m_4s_sq05_01 13 5 Forward 1-75m_4s_sq09_02 13 5 Forward 1-5m_4s_sq01_01 15 5 Forward 1-5m_4s_sq04_01 15 5 Forward 1-5m_4s_sq04_02 15 5 Forward 1-5m_4s_sq11_01 15 5 Forward 1-5m_4s_sq11_02 15 5 Forward 1-5m_4s_sq15_01 14 5 Forward 1-5m_4s_sq07_01 13 5 Forward


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.