Micro-Electron Diffraction data from Thaumatin, source of 5k7q structure

Data DOI: 10.15785/SBGRID/287 | ID: 287

Publication DOI: 10.1038/nmeth.4178

5k7q Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

EMDB 8219: RCSB PDBe

Gonen Laboratory, University of California, Los Angeles

Release Date: 31 Mar 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/287

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/287 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/287 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/287 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/287 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 287 ; shasum -c files.sha'

Storage requirements: 3.7G

Biological Sample:

Thaumatin

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Protein

Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

23 Mar 2015

Related Datasets:

None


Cite this Dataset

Hattne, J; de la Cruz, J; Gonen, T. 2017. "Micro-Electron Diffraction data for: Thaumatin. PDB Code 5k7q", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/287.

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Dataset Description

Micro-electron diffraction data from thaumatin

Project Members

Name Additional Roles Affiliation While Working on the Project
Johan HattneDepositorHHMI Janelia Research Campus
Jason de la CruzData CollectorHHMI Janelia Research Campus
Tamir GonenPIUniversity of California, Los Angeles

Reprocessing Instructions

Convert corrected images to SMV: $ img2px -d 2640 -s 11 -o movie_01/thau_###.img movie1-5m4s+2-_04_00?.tvips $ img2px -d 3600 -s 10 -o movie_06/thau_###.img movie2m4s-2+_sq11_01_00?.tvips $ img2px -d 3240 -s 12 -o movie_32/thau_###.img movie1-75m4s-2+_sq07_01_000.tvips MOSFLM processing parameters: DATASET ADC offset GAIN Direction of spindle rotation movie1-5m4s+2-_04 44 5 Backward movie2m4s-2+_sq11_01 41 5 Forward movie1-75m4s-2+_sq07_01 37 5 Forward


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.