X-Ray Diffraction data from citrate synthase (TpCS) with C-terminal His6 tag, source of 4YBO structure

Data DOI: 10.15785/SBGRID/283 | ID: 283

Publication DOI: 10.1107/S2053230X15015939

4YBO Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Kappock Laboratory, Purdue University

Release Date: May 27, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/283

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/283 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/283 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/283 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/283 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 283 ; shasum -c files.sha'

Storage requirements: 25G

Biological Sample:

citrate synthase (TpCS) with C-terminal His6 tag

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

beamline 21-ID-G, LS-CAT, APS, Chicago, IL

Data Creation Date:

July 2, 2010

Related Datasets:

None


Cite this Dataset

Donini, S; Kappock, T. 2016. "X-Ray Diffraction data for: citrate synthase (TpCS) with C-terminal His6 tag. PDB Code 4YBO", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/283.

Download Citation

Dataset Description

Structure of citrate synthase from the thermoacidophilic euryarchaeon Thermolasma acidophilum

Project Members

Name Additional Roles Affiliation While Working on the Project
Stefano DoniniData CollectorPurdue University
T. Joseph KappockDepositor, PIPurdue University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP3

Files:

/programs/datagrid/283/TpCS_v3.###

Images:

1 - 360

Beam:

(153.72, 147.99) -> (153.72, 147.93)

Distance:

250.0 -> 247.64

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.87 5.07 1.87
Low resolution limit 39.85 39.86 1.9
Completeness 97.7 99.3 96.3
Multiplicity 14.0 14.2 5.0
I/sigma 10.6 33.0 0.8
Rmerge 0.208 0.062 1.667
CC half 0.996 0.998 0.455
Anomalous completeness 97.7 99.5 95.2
Anomalous multiplicity 7.1 7.1 7.1
Cell 56.42 113.23 120.02 90.0 95.08 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP3

Files:

/programs/datagrid/283/TpCS_v3.###

Images:

1 - 360

Beam:

(153.72, 147.99) -> (153.72, 147.91)

Distance:

250.0 -> 249.64

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.89 5.13 1.89
Low resolution limit 40.14 40.15 1.92
Completeness 97.8 99.5 96.1
Multiplicity 13.7 14.1 4.9
I/sigma 10.7 33.0 0.9
Rmerge 0.204 0.06 1.454
CC half 0.997 0.998 0.525
Anomalous completeness 97.6 99.6 95.4
Anomalous multiplicity 6.9 6.9 6.9
Cell 56.48 113.33 120.07 90.0 95.11 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP3

Files:

/programs/datagrid/283/TpCS_v3.###

Images:

1 - 360

Beam:

(153.72, 147.99) -> (153.72, 148.01)

Distance:

250.0 -> 250.16

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.0 5.42 2.0
Low resolution limit 32.39 32.4 2.03
Completeness 97.8 99.0 92.2
Multiplicity 13.7 14.0 4.8
I/sigma 4.7 9.3 0.9
Rmerge 0.533 0.206 11.622
CC half 0.959 0.981 0.25
Anomalous completeness 97.3 99.3 86.7
Anomalous multiplicity 6.9 6.9 6.9
Cell 56.268 112.768 119.657 90.0 94.933 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.