X-Ray Diffraction data from WD40 domain of Human E3 Ubiquitin Ligase COP1 (RFWD2) bound to peptide from Trib1, source of 5IGQ structure

Data DOI: 10.15785/SBGRID/265 | ID: 265

Publication DOI: 10.1016/j.str.2016.03.002

5IGQ Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Blacklow Laboratory, Harvard Medical School

Release Date: May 27, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/265

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/265 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/265 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/265 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/265 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 265 ; shasum -c files.sha'

Storage requirements: 1.6G

Biological Sample:

WD40 domain of Human E3 Ubiquitin Ligase COP1 (RFWD2) bound to peptide from Trib1

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24 ID D

Data Creation Date:

April 10, 2014

Related Datasets:

None


Cite this Dataset

Uljon, S; Xu, X; Blacklow, S. 2016. "X-Ray Diffraction data for: WD40 domain of Human E3 Ubiquitin Ligase COP1 (RFWD2) bound to peptide from Trib1. PDB Code 5IGQ", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/265.

Download Citation

Dataset Description

single wavelength dataset of single crystal

Project Members

Name Additional Roles Affiliation While Working on the Project
Sacha UljonData Collector, DepositorBrigham and Women's Hospital
Xiang XuHMS
Stephen BlacklowPIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97921

Sweep:

SWEEP1

Files:

/programs/datagrid/265/2_08_06_1_###.img

Images:

1 - 90

Beam:

(152.07, 158.67) -> (152.07, 158.76)

Distance:

450.0 -> 453.88

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.99 10.81 3.99
Low resolution limit 58.72 58.73 4.06
Completeness 94.8 86.2 97.5
Multiplicity 2.0 2.0 2.0
I/sigma 5.4 15.3 0.9
Rmerge 0.094 0.04 1.71
CC half 0.993 0.992 0.442
Anomalous completeness 80.3 86.5 78.0
Anomalous multiplicity 1.1 1.1 1.1
Cell 175.69 124.64 176.17 90.0 90.46 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97921

Sweep:

SWEEP1

Files:

/programs/datagrid/265/2_08_06_1_###.img

Images:

1 - 90

Beam:

(152.07, 158.67) -> (152.07, 158.76)

Distance:

450.0 -> 453.32

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.87 10.5 3.87
Low resolution limit 124.89 124.96 3.94
Completeness 95.0 86.1 97.2
Multiplicity 2.0 2.0 2.0
I/sigma 5.0 15.3 0.9
Rmerge 0.103 0.041 1.332
CC half 0.993 0.992 0.32
Anomalous completeness 80.0 86.1 77.0
Anomalous multiplicity 1.1 1.1 1.1
Cell 175.68 124.64 176.16 90.0 90.46 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.