X-Ray Diffraction data from WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1, source of 5IGO structure

Data DOI: 10.15785/SBGRID/264 | ID: 264

Publication DOI: 10.1016/j.str.2016.03.002

5IGO Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Blacklow Laboratory, Harvard Medical School

Release Date: May 27, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/264

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/264 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/264 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/264 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/264 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 264 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24 ID C

Data Creation Date:

Dec. 17, 2015

Related Datasets:

None


Cite this Dataset

Uljon, S; Xu, X; Blacklow, S. 2016. "X-Ray Diffraction data for: WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1. PDB Code 5IGO", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/264.

Download Citation

Dataset Description

single wavelength x ray data set of single crystal

Project Members

Name Additional Roles Affiliation While Working on the Project
Sacha UljonData Collector, DepositorBrigham and Women's Hospital
Xiang XuHMS
Stephen BlacklowPIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9793

Sweep:

SWEEP1

Files:

/programs/datagrid/264/scb010_3_a_1_####.cbf

Images:

1 - 900

Beam:

(216.64, 222.44) -> (216.64, 222.74)

Distance:

300.0 -> 300.0

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.35 3.66 1.35
Low resolution limit 78.42 78.55 1.37
Completeness 82.1 97.2 30.5
Multiplicity 2.8 3.2 1.6
I/sigma 5.5 14.4 1.0
Rmerge 0.075 0.04 0.558
CC half 0.996 0.997 0.337
Anomalous completeness 65.6 89.7 10.9
Anomalous multiplicity 1.6 1.6 1.6
Cell 78.5 87.381 94.545 90.0 92.609 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9793

Sweep:

SWEEP1

Files:

/programs/datagrid/264/scb010_3_a_1_####.cbf

Images:

1 - 900

Beam:

(216.64, 222.44) -> (216.64, 222.57)

Distance:

300.0 -> 299.79

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.49 4.04 1.49
Low resolution limit 33.0 33.0 1.52
Completeness 96.2 97.8 77.2
Multiplicity 3.2 3.4 2.3
I/sigma 8.0 24.1 1.0
Rmerge 0.06 0.026 0.756
CC half 0.997 0.998 0.538
Anomalous completeness 86.2 90.6 53.2
Anomalous multiplicity 1.7 1.7 1.7
Cell 78.44 87.42 94.51 90.0 92.55 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9793

Sweep:

SWEEP1

Files:

/programs/datagrid/264/scb010_3_a_1_####.cbf

Images:

1 - 900

Beam:

(216.64, 222.44) -> (216.64, 222.57)

Distance:

300.0 -> 299.45

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.49 4.04 1.49
Low resolution limit 64.15 64.21 1.52
Completeness 96.2 97.9 77.2
Multiplicity 3.2 3.4 2.3
I/sigma 8.1 24.1 1.0
Rmerge 0.06 0.026 0.75
CC half 0.997 0.998 0.542
Anomalous completeness 86.3 90.6 53.3
Anomalous multiplicity 1.7 1.7 1.7
Cell 78.44 87.42 94.51 90.0 92.55 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9793

Sweep:

SWEEP1

Files:

/programs/datagrid/264/scb010_3_a_1_####.cbf

Images:

1 - 900

Beam:

(216.64, 222.44) -> (216.64, 222.56)

Distance:

300.0 -> 299.83

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.43 3.88 1.43
Low resolution limit 87.39 87.56 1.45
Completeness 92.8 98.7 58.5
Multiplicity 3.1 3.4 1.9
I/sigma 6.3 15.9 1.0
Rmerge 0.067 0.034 0.638
CC half 0.995 0.997 0.694
Anomalous completeness 81.1 93.9 32.3
Anomalous multiplicity 1.7 1.7 1.7
Cell 78.381 87.395 94.496 90.0 92.547 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.