Micro-Electron Diffraction data from Proteinase K, source of 5I9S structure

Data DOI: 10.15785/SBGRID/262 | ID: 262

Publication DOI: 10.1107/S1600576716007196

5I9S Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB


Gonen Laboratory, University of California, Los Angeles

Release Date: May 31, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/262

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/262 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/262 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/262 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/262 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 262 ; shasum -c files.sha'

Storage requirements: 1.7G

Biological Sample:

Proteinase K

Dataset Type:

Microcrystal Electron Diffraction

Subject Composition:


Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

Dec. 7, 2015

Related Datasets:


Cite this Dataset

Johan, H; Shi, D; Gonen, T. 2016. "Microcrystal Electron Diffraction data for: Proteinase K. PDB Code 5I9S", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/262.

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Dataset Description

MicroED structure of proteinase K at 1.75 A resolution

Project Members

Name Additional Roles Affiliation While Working on the Project
Hattne JohanDepositorHHMI Janelia Research Campus
Dan ShiData CollectorHHMI Janelia Research Campus
Tamir GonenPIUniversity of California, Los Angeles

Reprocessing Instructions

Convert corrected images to SMV: $ img2px -d 2220 -s 5.6 -o movie_14_01_corr/prot_###.img cl1200mm4sp14mv01_000.tvips $ img2px -d 2200 -s 5.2 -o movie_18_01_corr/prot_###.img cl1200mm4sp18mv01_000.tvips $ img2px -d 2200 -s 5.7 -o movie_20_01_corr/prot_###.img cl1200mm4sp20mv01_000.tvips $ img2px -d 2200 -s 5.6 -o movie_21_01_corr/prot_###.img cl1200mm4sp21mv01_000.tvips MOSFLM processing parameters: DATASET ADC offset GAIN Direction of spindle rotation cl1200mm4sp14mv01_000.tvips 14 5 Forward cl1200mm4sp18mv01_000.tvips 15 5 Backward cl1200mm4sp20mv01_000.tvips 14 5 Forward cl1200mm4sp21mv01_000.tvips 14 5 Forward

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.