Data DOI: 10.15785/SBGRID/262 | ID: 262
Publication DOI: 10.1107/S1600576716007196
5I9S Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Gonen Laboratory, University of California, Los Angeles
Release Date: 31 May 2016
1. If this dataset is locally available, it should be accessable at /programs/datagrid/262
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/262 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/262 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/262 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/262 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 262 ; shasum -c files.sha'
Storage requirements: 1.7G
Biological Sample:
Proteinase K
Dataset Type:
Microcrystal Electron Diffraction
Subject Composition:
Protein
Collection Facility:
FEI TF20 (Janelia Research Campus)
Data Creation Date:
7 Dec 2015
Related Datasets:
None
Johan, H; Shi, D; Gonen, T. 2016. "Microcrystal Electron Diffraction data for: Proteinase K. PDB Code 5I9S", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/262.
MicroED structure of proteinase K at 1.75 A resolution
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Hattne Johan | Depositor | HHMI Janelia Research Campus |
| Dan Shi | Data Collector | HHMI Janelia Research Campus |
| Tamir Gonen | PI | University of California, Los Angeles |
Convert corrected images to SMV: $ img2px -d 2220 -s 5.6 -o movie_14_01_corr/prot_###.img cl1200mm4sp14mv01_000.tvips $ img2px -d 2200 -s 5.2 -o movie_18_01_corr/prot_###.img cl1200mm4sp18mv01_000.tvips $ img2px -d 2200 -s 5.7 -o movie_20_01_corr/prot_###.img cl1200mm4sp20mv01_000.tvips $ img2px -d 2200 -s 5.6 -o movie_21_01_corr/prot_###.img cl1200mm4sp21mv01_000.tvips MOSFLM processing parameters: DATASET ADC offset GAIN Direction of spindle rotation cl1200mm4sp14mv01_000.tvips 14 5 Forward cl1200mm4sp18mv01_000.tvips 15 5 Backward cl1200mm4sp20mv01_000.tvips 14 5 Forward cl1200mm4sp21mv01_000.tvips 14 5 Forward
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
