X-Ray Diffraction data from S. cerevisiae Csm1 69-190 L157M mutant, Selenomethionine-labeled, source of 3N4R structure

Data DOI: 10.15785/SBGRID/26 | ID: 26

Publication DOI: 10.1016/j.cell.2010.07.017

3N4R Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Harrison Laboratory, Harvard Medical School

Release Date: May 6, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/26

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/26 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/26 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/26 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/26 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 26 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

S. cerevisiae Csm1 69-190 L157M mutant, Selenomethionine-labeled

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Advanced Photon Source BL 24ID-C

Data Creation Date:

Aug. 2, 2008

Related Datasets:

None


Cite this Dataset

Corbett, KD; Harrison, S. 2015. "X-Ray Diffraction data for: S. cerevisiae Csm1 69-190 L157M mutant, Selenomethionine-labeled. PDB Code 3N4R", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/26.

Download Citation

Dataset Description

Two-wedge SeMet peak wavelength dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettData Collector, DepositorHarvard Medical School
Stephen HarrisonPIHarvard Medical School

Reprocessing Instructions

Process together in HKL2000 to 2.6 Å

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP2

Files:

/programs/datagrid/26/kc66_4_3_###.img

Images:

1 - 90

Beam:

(157.2, 156.5) -> (157.2, 156.52)

Distance:

350.0 -> 351.32

Spacegroup:

H 3

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.42 6.57 2.42
Low resolution limit 61.18 61.2 2.46
Completeness 99.9 99.1 99.9
Multiplicity 5.4 5.7 2.8
I/sigma 11.6 29.8 1.1
Rmerge 0.07 0.036 0.93
CC half 0.998 0.998 0.463
Anomalous completeness 99.3 98.5 94.2
Anomalous multiplicity 2.7 2.7 2.7
Cell 72.571 72.571 267.34 90.0 90.0 120.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP2

Files:

/programs/datagrid/26/kc66_4_3_###.img

Images:

1 - 90

Beam:

(157.2, 156.5) -> (157.2, 156.97)

Distance:

350.0 -> 350.51

Spacegroup:

P 31

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.79 7.57 2.79
Low resolution limit 62.74 62.76 2.84
Completeness 99.7 99.9 99.3
Multiplicity 5.5 5.2 4.0
I/sigma 7.6 17.7 1.1
Rmerge 0.082 0.035 1.014
CC half 0.998 0.997 0.804
Anomalous completeness 98.4 97.5 96.1
Anomalous multiplicity 2.8 2.8 2.8
Cell 72.45 72.45 267.04 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP2

Files:

/programs/datagrid/26/kc66_4_3_###.img

Images:

1 - 90

Beam:

(157.2, 156.5) -> (157.2, 156.95)

Distance:

350.0 -> 349.24

Spacegroup:

H 3

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.47 6.7 2.47
Low resolution limit 56.6 56.62 2.51
Completeness 100.0 99.1 99.9
Multiplicity 5.6 5.8 3.6
I/sigma 11.9 28.8 1.2
Rmerge 0.062 0.031 0.863
CC half 0.998 0.999 0.646
Anomalous completeness 99.6 98.3 95.6
Anomalous multiplicity 2.8 2.8 2.8
Cell 72.216 72.216 266.149 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.