Data DOI: 10.15785/SBGRID/26 | ID: 26
Publication DOI: 10.1016/j.cell.2010.07.017
3N4R Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Harrison Laboratory, Harvard Medical School
Release Date: 6 May 2015
1. If this dataset is locally available, it should be accessable at /programs/datagrid/26
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/26 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/26 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/26 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/26 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 26 ; shasum -c files.sha'
Storage requirements: 3.2G
Biological Sample:
S. cerevisiae Csm1 69-190 L157M mutant, Selenomethionine-labeled
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
Advanced Photon Source BL 24ID-C
Data Creation Date:
2 Aug 2008
Related Datasets:
None
Corbett, KD; Harrison, S. 2015. "X-Ray Diffraction data for: S. cerevisiae Csm1 69-190 L157M mutant, Selenomethionine-labeled. PDB Code 3N4R", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/26.
Two-wedge SeMet peak wavelength dataset
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Kevin D Corbett | Data Collector, Depositor | Harvard Medical School |
| Stephen Harrison | PI | Harvard Medical School |
Process together in HKL2000 to 2.6 Å
Version:
version unreported
Reprocessing failed.
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97949
Sweep:
SWEEP2
Files:
/programs/datagrid/26/kc66_4_3_###.img
Images:
1 - 90
Beam:
(157.2, 156.5) -> (157.2, 156.97)
Distance:
350.0 -> 350.51
Spacegroup:
P 31
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.54 | 6.89 | 2.54 |
| Low resolution limit | 61.07 | 61.09 | 2.58 |
| Completeness | 99.8 | 99.7 | 99.9 |
| Multiplicity | 5.5 | 5.2 | 3.2 |
| I/sigma | 6.0 | 17.3 | 0.4 |
| Rmerge | 0.102 | 0.035 | 2.312 |
| CC half | 0.998 | 0.998 | 0.34 |
| Anomalous completeness | 98.5 | 97.1 | 94.9 |
| Anomalous multiplicity | 2.8 | 2.8 | 2.8 |
| Cell | 72.44 | 72.44 | 267.01 | 90.0 | 90.0 | 120.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97949
Sweep:
SWEEP2
Files:
/programs/datagrid/26/kc66_4_3_###.img
Images:
1 - 90
Beam:
(157.2, 156.5) -> (157.2, 156.95)
Distance:
350.0 -> 349.14
Spacegroup:
R 3
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.31 | 6.27 | 2.31 |
| Low resolution limit | 56.6 | 56.62 | 2.35 |
| Completeness | 100.0 | 99.9 | 99.3 |
| Multiplicity | 5.8 | 5.8 | 5.4 |
| I/sigma | 12.2 | 55.5 | 0.2 |
| Rmerge | 0.073 | 0.034 | 2.248 |
| CC half | 0.995 | 0.979 | 0.398 |
| Anomalous completeness | 99.9 | 99.9 | 99.3 |
| Anomalous multiplicity | 2.9 | 2.9 | 2.9 |
| Cell | 72.217 | 72.217 | 266.172 | 90.0 | 90.0 | 120.0 |
|---|
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
