X-Ray Diffraction data from Drosophila Dpr6 D1 bound to DIPalpha D1+D2, source of 5EO9 structure

Data DOI: 10.15785/SBGRID/254 | ID: 254

Publication DOI: 10.1016/j.cell.2015.11.022

5EO9 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Ozkan Laboratory, University of Chicago

Release Date: April 12, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/254

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/254 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/254 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/254 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/254 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 254 ; shasum -c files.sha'

Storage requirements: 11G

Biological Sample:

Drosophila Dpr6 D1 bound to DIPalpha D1+D2

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Beamline 11-1, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA

Data Creation Date:

May 18, 2013

Related Datasets:

None


Cite this Dataset

Garcia, K; Ozkan, E. 2016. "X-Ray Diffraction data for: Drosophila Dpr6 D1 bound to DIPalpha D1+D2. PDB Code 5EO9", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/254.

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Dataset Description

High-resolution native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
K. Christopher GarciaStanford University
Engin OzkanData Collector, Depositor, PIUniversity of Chicago

Reprocessing Instructions

HKL2000 beam center parameters should be x beam, y beam = 216.76, 212.26. XDS beam center parameters are in the header.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97945

Sweep:

SWEEP2

Files:

/programs/datagrid/254/C1_2_#####.cbf

Images:

1 - 900

Beam:

(216.63, 212.16) -> (216.63, 212.06)

Distance:

450.0 -> 450.07

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.19 5.94 2.19
Low resolution limit 129.99 130.22 2.23
Completeness 99.5 100.0 87.1
Multiplicity 10.3 10.6 5.7
I/sigma 11.1 24.9 1.2
Rmerge 0.116 0.071 1.228
CC half 0.998 0.998 0.466
Anomalous completeness 98.9 100.0 80.1
Anomalous multiplicity 5.6 5.6 5.6
Cell 129.988 49.328 54.585 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97945

Sweep:

SWEEP2

Files:

/programs/datagrid/254/C1_2_#####.cbf

Images:

1 - 900

Beam:

(216.63, 212.16) -> (216.63, 211.96)

Distance:

450.0 -> 453.35

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.19 5.94 2.19
Low resolution limit 35.37 35.37 2.23
Completeness 99.9 100.0 99.9
Multiplicity 11.2 11.2 6.4
I/sigma 16.8 45.3 1.3
Rmerge 0.076 0.046 1.304
CC half 0.999 0.999 0.533
Anomalous completeness 99.7 100.0 98.8
Anomalous multiplicity 6.0 6.0 6.0
Cell 130.61 49.47 54.87 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97945

Sweep:

SWEEP2

Files:

/programs/datagrid/254/C1_2_#####.cbf

Images:

1 - 900

Beam:

(216.63, 212.16) -> (216.63, 211.99)

Distance:

450.0 -> 450.37

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.17 5.88 2.17
Low resolution limit 54.95 54.97 2.2
Completeness 89.9 99.2 16.7
Multiplicity 3.1 3.4 1.8
I/sigma 11.1 30.2 1.1
Rmerge 0.046 0.025 0.415
CC half 0.998 0.999 0.672
Anomalous completeness 73.5 91.1 11.3
Anomalous multiplicity 1.7 1.7 1.7
Cell 49.527 54.953 130.817 90.004 89.998 89.942

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.