X-Ray Diffraction data from Protective Human Antibodies 100 and 114 in Complex with Ebola Virus Fusion Glycoprotein (GP), source of 5FHC structure

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Data DOI: 10.15785/SBGRID/250 | ID: 250

Publication DOI: 10.1126/science.aad6117

5FHC Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

McLellan Laboratory, University of Texas at Austin

Release Date: 1 Apr 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/250

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/250 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/250 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/250 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/250 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 250 ; shasum -c files.sha'

Storage requirements: 6.8G

Biological Sample:

Protective Human Antibodies 100 and 114 in Complex with Ebola Virus Fusion Glycoprotein (GP)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

19-ID, SBC, APS, Chicago, IL

Data Creation Date:

24 Oct 2014

Related Datasets:

None


Cite this Dataset

Gilman, MSA; McLellan, J. 2016. "X-Ray Diffraction data for: Protective Human Antibodies 100 and 114 in Complex with Ebola Virus Fusion Glycoprotein (GP). PDB Code 5FHC", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/250.

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Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Morgan SA GilmanData Collector, DepositorGeisel School of Medicine at Dartmouth
Jason McLellanPIUniversity of Texas at Austin

Reprocessing Instructions

Beam center: X beam=153.1, Y beam=162.4 Processing with iMosflm will require "Reverse direction of spindle rotation" to be checked

Reprocessing Data

About Reprocessing

xia2 -2d

xia2 -3d

xia2 -3dii

xia2 -dials
/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=3d mosflm_beam_centre=153.17,162.30 /programs/datagrid//250/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP2

Files:

/programs/datagrid/250/Zdm114_X1_P4P4b.####.img

Images:

1 - 360

Beam:

(153.17, 162.3) -> (153.17, 162.42)

Distance:

711.26 -> 717.93

Spacegroup:

H 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 6.12 16.32 6.12
Low resolution limit 42.91 42.91 6.23
Completeness 89.0 99.3 29.7
Multiplicity 10.6 9.3 2.1
I/sigma 7.1 20.1 0.7
Rmerge 0.271 0.089 1.004
CC half 0.993 0.993 0.12
Anomalous completeness 86.6 98.0 20.4
Anomalous multiplicity 5.8 5.8 5.8
Cell 171.63 171.63 383.52 90.0 90.0 120.0
/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=3dii mosflm_beam_centre=153.17,162.30 /programs/datagrid//250/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Reprocessing failed.

/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=dials mosflm_beam_centre=153.17,162.30 /programs/datagrid//250/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.