X-Ray Diffraction data from SrpA R34E variant, source of 5EQ4 structure

Data DOI: 10.15785/SBGRID/243 | ID: 243

5EQ4 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Iverson Laboratory, Vanderbilt University

Release Date: April 1, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/243

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/243 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/243 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/243 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/243 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 243 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

SrpA R34E variant

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 21-ID-G

Data Creation Date:

Aug. 22, 2015

Related Datasets:

None


Cite this Dataset

Loukachevitch, LV; Iverson, TM. 2016. "X-Ray Diffraction data for: SrpA R34E variant. PDB Code 5EQ4", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/243.

Download Citation

Dataset Description

point mutant that wrecks ligand binding

Project Members

Name Additional Roles Affiliation While Working on the Project
Tina M IversonDepositorVanderbilt
Lioudmila V LoukachevitchData CollectorVanderbilt
Tina IversonPIVanderbilt University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/243/srpasur347e_cr28_1_1_10.###

Images:

1 - 360

Beam:

(145.37, 155.42) -> (145.37, 155.56)

Distance:

350.0 -> 349.79

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.04 5.54 2.04
Low resolution limit 88.04 88.13 2.08
Completeness 84.2 100.0 40.1
Multiplicity 12.5 13.1 8.3
I/sigma 11.2 23.6 1.2
Rmerge 0.167 0.061 1.771
CC half 0.996 0.999 0.267
Anomalous completeness 83.2 100.0 41.0
Anomalous multiplicity 6.7 6.7 6.7
Cell 44.725 88.044 199.383 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/243/srpasur347e_cr28_1_1_10.###

Images:

1 - 360

Beam:

(145.37, 155.42) -> (145.37, 155.49)

Distance:

350.0 -> 349.16

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.06 5.59 2.06
Low resolution limit 43.99 44.01 2.1
Completeness 88.8 100.0 48.5
Multiplicity 13.1 12.8 8.8
I/sigma 15.9 41.8 1.2
Rmerge 0.12 0.042 1.516
CC half 0.999 0.999 0.401
Anomalous completeness 89.1 99.6 49.7
Anomalous multiplicity 6.9 6.9 6.9
Cell 44.72 87.99 199.14 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/243/srpasur347e_cr28_1_1_10.###

Images:

1 - 360

Beam:

(145.37, 155.42) -> (145.37, 155.5)

Distance:

350.0 -> 349.4

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.06 5.59 2.06
Low resolution limit 49.78 49.8 2.1
Completeness 88.8 100.0 48.5
Multiplicity 13.1 12.8 8.8
I/sigma 15.9 41.8 1.2
Rmerge 0.12 0.042 1.506
CC half 0.999 0.999 0.41
Anomalous completeness 89.1 99.6 49.7
Anomalous multiplicity 6.9 6.9 6.9
Cell 44.72 87.99 199.14 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/243/srpasur347e_cr28_1_1_10.###

Images:

1 - 360

Beam:

(145.37, 155.42) -> (145.37, 155.59)

Distance:

350.0 -> 348.67

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.08 5.64 2.08
Low resolution limit 99.59 99.7 2.12
Completeness 89.5 100.0 48.6
Multiplicity 13.2 13.0 8.8
I/sigma 10.0 18.6 1.3
Rmerge 0.16 0.063 1.787
CC half 0.995 0.997 0.593
Anomalous completeness 89.9 100.0 50.4
Anomalous multiplicity 7.0 7.0 7.0
Cell 44.723 88.022 199.173 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.