Data DOI: 10.15785/SBGRID/233 | ID: 233
Publication DOI: 10.7554/eLife.12411
5HAS Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Fromme Laboratory, Cornell University
Release Date: 11 Mar 2016
1. If this dataset is locally available, it should be accessable at /programs/datagrid/233
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/233 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/233 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/233 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/233 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 233 ; shasum -c files.sha'
Storage requirements: 2.9G
Biological Sample:
Sec7 (T. terrestris) DCB/HUS domain
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
CHESS A1
Data Creation Date:
8 May 2013
Related Datasets:
Richardson, BC.; Fromme, J. 2016. "X-Ray Diffraction data for: Sec7 (T. terrestris) DCB/HUS domain. PDB Code 5HAS", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/233.
SAD data of SeMet protein crystals. Data was collected in 10-image wedges using an inverse beam strategy.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Brian C. Richardson | Data Collector | Cornell University |
| J. Christopher Fromme | Data Collector, Depositor, PI | Cornell University |
We used frames 1-149 (in both direct and inverse beam orientations). Later frames were excluded due to radiation damage and loss of anomalous signal.
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.9779
Sweep:
SWEEP2
Files:
/programs/datagrid/233/FL01D_101_###.img
Images:
1 - 180
Beam:
(101.09, 109.56) -> (101.09, 109.27)
Distance:
275.0 -> 275.77
Spacegroup:
P 21 21 2
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.62 | 7.01 | 2.62 |
| Low resolution limit | 19.68 | 19.68 | 2.67 |
| Completeness | 100.0 | 100.0 | 100.0 |
| Multiplicity | 13.8 | 12.9 | 7.9 |
| I/sigma | 11.6 | 34.7 | 1.0 |
| Rmerge | 0.145 | 0.05 | 2.057 |
| CC half | 0.999 | 0.999 | 0.372 |
| Anomalous completeness | 99.9 | 99.6 | 99.8 |
| Anomalous multiplicity | 7.3 | 7.3 | 7.3 |
| Cell | 247.86 | 62.49 | 132.07 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.9779
Sweep:
SWEEP2
Files:
/programs/datagrid/233/FL01D_101_###.img
Images:
1 - 180
Beam:
(101.09, 109.56) -> (101.09, 109.27)
Distance:
275.0 -> 275.24
Spacegroup:
P 21 21 2
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 2.62 | 7.11 | 2.62 |
| Low resolution limit | 61.97 | 61.98 | 2.67 |
| Completeness | 100.0 | 100.0 | 100.0 |
| Multiplicity | 13.7 | 12.5 | 6.6 |
| I/sigma | 11.5 | 32.8 | 0.9 |
| Rmerge | 0.144 | 0.05 | 1.999 |
| CC half | 0.999 | 0.998 | 0.354 |
| Anomalous completeness | 100.0 | 99.7 | 99.8 |
| Anomalous multiplicity | 7.2 | 7.2 | 7.2 |
| Cell | 247.86 | 62.49 | 132.07 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Reprocessing failed.
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
