X-Ray Diffraction data from Sec7 (T. terrestris) DCB/HUS domain, source of 5HAS structure

Data DOI: 10.15785/SBGRID/233 | ID: 233

Publication DOI: 10.7554/eLife.12411

5HAS Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Fromme Laboratory, Cornell University

Release Date: March 11, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/233

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/233 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/233 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/233 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/233 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 233 ; shasum -c files.sha'

Storage requirements: 2.9G

Biological Sample:

Sec7 (T. terrestris) DCB/HUS domain

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

CHESS A1

Data Creation Date:

May 8, 2013

Related Datasets:

234


Cite this Dataset

Richardson, BC.; Fromme, J. 2016. "X-Ray Diffraction data for: Sec7 (T. terrestris) DCB/HUS domain. PDB Code 5HAS", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/233.

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Dataset Description

SAD data of SeMet protein crystals. Data was collected in 10-image wedges using an inverse beam strategy.

Project Members

Name Additional Roles Affiliation While Working on the Project
Brian C. RichardsonData CollectorCornell University
J. Christopher FrommeData Collector, Depositor, PICornell University

Reprocessing Instructions

We used frames 1-149 (in both direct and inverse beam orientations). Later frames were excluded due to radiation damage and loss of anomalous signal.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9779

Sweep:

SWEEP2

Files:

/programs/datagrid/233/FL01D_101_###.img

Images:

1 - 180

Beam:

(101.09, 109.56) -> (101.09, 109.27)

Distance:

275.0 -> 275.94

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.7 7.21 2.7
Low resolution limit 19.68 19.68 2.75
Completeness 100.0 100.0 100.0
Multiplicity 14.2 13.0 10.0
I/sigma 12.4 34.5 1.3
Rmerge 0.139 0.05 1.721
CC half 0.999 0.999 0.497
Anomalous completeness 99.9 99.3 99.8
Anomalous multiplicity 7.5 7.5 7.5
Cell 247.91 62.5 132.09 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9779

Sweep:

SWEEP2

Files:

/programs/datagrid/233/FL01D_101_###.img

Images:

1 - 180

Beam:

(101.09, 109.56) -> (101.09, 109.27)

Distance:

275.0 -> 275.24

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.71 7.35 2.71
Low resolution limit 61.98 61.99 2.76
Completeness 100.0 100.0 100.0
Multiplicity 14.2 12.5 10.1
I/sigma 12.5 32.5 1.3
Rmerge 0.138 0.05 1.7
CC half 0.999 0.999 0.509
Anomalous completeness 99.9 99.3 99.9
Anomalous multiplicity 7.5 7.5 7.5
Cell 247.91 62.5 132.09 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.