X-Ray Diffraction data from Snf7 core (ESCRT-III filament), source of 5FD9 structure

Data DOI: 10.15785/SBGRID/232 | ID: 232

Publication DOI: 10.7554/eLife.12548

5FD9 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Fromme Laboratory, Cornell University

Release Date: March 11, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/232

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/232 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/232 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/232 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/232 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 232 ; shasum -c files.sha'

Storage requirements: 1.8G

Biological Sample:

Snf7 core (ESCRT-III filament)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

CHESS F1

Data Creation Date:

March 26, 2015

Related Datasets:

None


Cite this Dataset

Emr, SD.; Mao, Y; Tang, S; Fromme, J. 2016. "X-Ray Diffraction data for: Snf7 core (ESCRT-III filament). PDB Code 5FD9", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/232.

Download Citation

Dataset Description

Data collected on Snf7 filament crystals after high-pressure freezing.

Project Members

Name Additional Roles Affiliation While Working on the Project
Scott D. EmrCornell University
Yuxin MaoCornell University
Shaogang TangData CollectorCornell University
J. Christopher FrommeData Collector, Depositor, PICornell University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9804

Sweep:

SWEEP1

Files:

/programs/datagrid/232/hione_2_###.img

Images:

1 - 210

Beam:

(138.99, 130.94) -> (138.99, 130.5)

Distance:

175.0 -> 175.63

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.55 4.2 1.55
Low resolution limit 20.62 20.62 1.58
Completeness 96.1 92.5 94.0
Multiplicity 4.4 3.9 4.4
I/sigma 10.7 24.6 1.1
Rmerge 0.058 0.043 1.161
CC half 0.996 0.993 0.453
Anomalous completeness 94.3 86.8 90.0
Anomalous multiplicity 2.3 2.3 2.3
Cell 29.9 46.24 44.61 90.0 98.5 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9804

Sweep:

SWEEP1

Files:

/programs/datagrid/232/hione_2_###.img

Images:

1 - 210

Beam:

(138.99, 130.94) -> (138.99, 130.5)

Distance:

175.0 -> 175.56

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.55 4.2 1.55
Low resolution limit 31.92 31.93 1.58
Completeness 96.1 92.5 94.0
Multiplicity 4.4 3.9 4.4
I/sigma 10.7 24.5 1.1
Rmerge 0.058 0.043 1.161
CC half 0.996 0.992 0.432
Anomalous completeness 94.3 86.6 90.3
Anomalous multiplicity 2.3 2.3 2.3
Cell 29.9 46.24 44.61 90.0 98.5 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.