Micro-Electron Diffraction data from Lysozyme, source of 3J6K structure

Data DOI: 10.15785/SBGRID/222 | ID: 222

Publication DOI: 10.1038/nmeth.3043

3J6K Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Gonen Laboratory, University of California, Los Angeles

Release Date: March 4, 2016

Supersedes: 10.15785/SBGRID/185

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/222

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/222 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/222 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/222 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/222 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 222 ; shasum -c files.sha'

Storage requirements: 1.8G

Biological Sample:

Lysozyme

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Protein

Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

Feb. 4, 1914

Related Datasets:

185


Cite this Dataset

Shi, D; Gonen, T. 2016. "Micro-Electron Diffraction data for: Lysozyme. PDB Code 3J6K", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/222.

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Dataset Description

Lysozyme microcrystals

Project Members

Name Additional Roles Affiliation While Working on the Project
Dan ShiData Collector, DepositorHHMI Janelia Research Campus
Tamir GonenPIUniversity of California, Los Angeles

Reprocessing Instructions

Convert the images to SMV: $ tvips2smv -d 2640 -r 0.09 -o movie4s_01/movie4s_01_###.img 185/movie4s_01_00?.tvips $ tvips2smv -d 2640 -r 0.09 -o movie4s_23/movie4s_23_###.img 185/movie4s_23_00?.tvips MOSFLM processing parameters (all datasets): ADC offset: 0 Direction of spindle rotation: Reverse Gain: 2


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.