X-Ray Diffraction data from Prefusion RSV F Glycoprotein In Complex with Inhibitor BMS-433771, source of 5EA7 structure

Data DOI: 10.15785/SBGRID/216 | ID: 216

Publication DOI: 10.1038/nchembio.1982

5EA7 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

McLellan Laboratory, University of Texas at Austin

Release Date: Jan. 8, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/216

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/216 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/216 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/216 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/216 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 216 ; shasum -c files.sha'

Storage requirements: 2.7G

Biological Sample:

Prefusion RSV F Glycoprotein In Complex with Inhibitor BMS-433771

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

APS BEAMLINE 19-ID

Data Creation Date:

July 19, 2014

Related Datasets:

None


Cite this Dataset

Battles, MB; McLellan, JS. 2016. "X-Ray Diffraction data for: Prefusion RSV F Glycoprotein In Complex with Inhibitor BMS-433771. PDB Code 5EA7", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/216.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Michael B BattlesData Collector, DepositorGeisel School of Medicine at Dartmouth
Jason S McLellanGeisel School of Medicine at Dartmouth
Jason McLellanPIUniversity of Texas at Austin

Reprocessing Instructions

Beam center, as defined in the image headers, needs to be corrected to X=153.1 Y=162.5 Processing with iMosflm will require "Reverse direction of spindle rotation" to be checked

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/216/F_1401_X1_P3P3.####.img

Images:

1 - 150

Beam:

(154.5, 159.98) -> (154.5, 162.51)

Distance:

463.28 -> 459.71

Spacegroup:

P 43 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.84 7.71 2.84
Low resolution limit 56.74 56.75 2.89
Completeness 100.0 100.0 100.0
Multiplicity 16.8 14.4 11.8
I/sigma 14.7 39.4 1.0
Rmerge 0.176 0.047 2.86
CC half 0.999 0.999 0.354
Anomalous completeness 99.7 99.9 100.0
Anomalous multiplicity 9.1 9.1 9.1
Cell 170.21 170.21 170.21 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/216/F_1401_X1_P3P3.####.img

Images:

1 - 150

Beam:

(154.5, 159.98) -> (154.5, 162.51)

Distance:

463.28 -> 461.68

Spacegroup:

P 43 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.85 7.73 2.85
Low resolution limit 56.74 56.75 2.9
Completeness 100.0 100.0 100.0
Multiplicity 16.8 14.4 12.1
I/sigma 14.9 39.3 1.0
Rmerge 0.175 0.047 2.808
CC half 0.999 0.999 0.326
Anomalous completeness 99.7 99.7 100.0
Anomalous multiplicity 9.2 9.2 9.2
Cell 170.21 170.21 170.21 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/216/F_1401_X1_P3P3.####.img

Images:

1 - 150

Beam:

(154.5, 159.98) -> (154.5, 162.52)

Distance:

463.28 -> 458.92

Spacegroup:

P 43 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.97 8.05 2.97
Low resolution limit 56.68 56.69 3.02
Completeness 100.0 100.0 100.0
Multiplicity 17.0 14.6 15.2
I/sigma 8.8 16.3 1.7
Rmerge 0.304 0.08 3.937
CC half 0.992 0.993 0.734
Anomalous completeness 100.0 99.8 100.0
Anomalous multiplicity 9.3 9.3 9.3
Cell 170.041 170.041 170.041 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.