X-Ray Diffraction data from Mesorhizobium opportunistum ParD3 antitoxin bound to ParE3 toxin, source of 5CEG structure

Data DOI: 10.15785/SBGRID/198 | ID: 198

Publication DOI: 10.1016/j.cell.2015.09.055

5CEG Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Crosson Laboratory, University of Chicago

Release Date: Nov. 24, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/198

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/198 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/198 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/198 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/198 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 198 ; shasum -c files.sha'

Storage requirements: 6.9G

Biological Sample:

Mesorhizobium opportunistum ParD3 antitoxin bound to ParE3 toxin

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Advanced Photon Source beamline 21-ID-D

Data Creation Date:

Dec. 14, 2014

Related Datasets:

None


Cite this Dataset

Aakre, C; Herrou, J; Laub, M; Crosson, S. 2015. "X-Ray Diffraction data for: Mesorhizobium opportunistum ParD3 antitoxin bound to ParE3 toxin. PDB Code 5CEG", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/198.

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Dataset Description

Iodine SAD dataset collected at 12670 electron volts. Crystal diffraction was measured at a temperature of 100 K using a 1 degree oscillation range.

Project Members

Name Additional Roles Affiliation While Working on the Project
Chris AakreMIT
Julien HerrouData CollectorUniversity of Chicago
Mike LaubMIT
Sean CrossonData Collector, Depositor, PIUniversity of Chicago

Reprocessing Instructions

Indexed in I222.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/198/Tat53.###

Images:

1 - 220

Beam:

(145.21, 155.63) -> (145.21, 155.7)

Distance:

170.0 -> 170.29

Spacegroup:

I 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.4 3.8 1.4
Low resolution limit 52.86 52.89 1.42
Completeness 99.9 99.7 99.3
Multiplicity 8.9 8.6 7.9
I/sigma 8.4 20.7 1.2
Rmerge 0.126 0.08 1.814
CC half 0.995 0.992 0.319
Anomalous completeness 99.3 98.8 97.4
Anomalous multiplicity 4.6 4.6 4.6
Cell 43.174 118.838 211.421 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/198/Tat53.###

Images:

1 - 220

Beam:

(145.21, 155.63) -> (145.21, 155.61)

Distance:

170.0 -> 170.08

Spacegroup:

I 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.41 3.83 1.41
Low resolution limit 40.58 40.6 1.43
Completeness 100.0 99.8 100.0
Multiplicity 9.0 8.5 8.5
I/sigma 9.8 25.3 0.9
Rmerge 0.11 0.072 2.066
CC half 0.996 0.994 0.314
Anomalous completeness 99.6 99.6 99.8
Anomalous multiplicity 4.7 4.7 4.7
Cell 43.18 118.84 211.41 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/198/Tat53.###

Images:

1 - 220

Beam:

(145.21, 155.63) -> (145.21, 155.61)

Distance:

170.0 -> 170.11

Spacegroup:

I 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.42 3.85 1.42
Low resolution limit 52.85 52.89 1.44
Completeness 100.0 99.8 100.0
Multiplicity 9.0 8.5 8.8
I/sigma 10.0 25.3 1.1
Rmerge 0.109 0.072 1.906
CC half 0.996 0.994 0.352
Anomalous completeness 99.6 99.6 99.9
Anomalous multiplicity 4.7 4.7 4.7
Cell 43.18 118.84 211.41 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/198/Tat53.###

Images:

1 - 220

Beam:

(145.21, 155.63) -> (145.21, 155.7)

Distance:

170.0 -> 170.17

Spacegroup:

I 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.42 3.85 1.42
Low resolution limit 40.58 40.59 1.44
Completeness 100.0 100.0 99.8
Multiplicity 9.0 8.6 8.4
I/sigma 8.7 21.3 1.1
Rmerge 0.118 0.078 2.335
CC half 0.996 0.993 0.404
Anomalous completeness 100.0 99.8 99.7
Anomalous multiplicity 4.7 4.7 4.7
Cell 43.171 118.823 211.408 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.