X-Ray Diffraction data from Brucella abortus RicA protein, source of 4N27 structure

Data DOI: 10.15785/SBGRID/197 | ID: 197

Publication DOI: 10.1021/bi401373r

4N27 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Crosson Laboratory, University of Chicago

Release Date: Nov. 24, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/197

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/197 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/197 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/197 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/197 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 197 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Brucella abortus RicA protein

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

beamline 21-ID-F (LS-CAT), Advanced Photon Source, Argonne, Illinois

Data Creation Date:

May 30, 2013

Related Datasets:

None


Cite this Dataset

Herrou, J; Crosson, S. 2015. "X-Ray Diffraction data for: Brucella abortus RicA protein. PDB Code 4N27", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/197.

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Dataset Description

Diffraction data from this crystal of B. abortus RicA were collected at a temperature of 100 K using a 1° oscillation range.

Project Members

Name Additional Roles Affiliation While Working on the Project
Julien HerrouData CollectorUniversity of Chicago
Sean CrossonData Collector, Depositor, PIUniversity of Chicago

Reprocessing Instructions

Images were collected on a MAR Mosaic 225 detector. Indexed in P21.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/197/RicA1.###

Images:

1 - 180

Beam:

(112.38, 111.95) -> (112.38, 112.0)

Distance:

200.0 -> 201.5

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.4 6.51 2.4
Low resolution limit 52.99 53.0 2.44
Completeness 98.2 99.3 97.2
Multiplicity 2.0 2.0 2.0
I/sigma 2.7 5.3 0.8
Rmerge 0.305 0.108 3.483
CC half 0.953 0.962 0.441
Anomalous completeness 84.2 91.0 79.1
Anomalous multiplicity 1.1 1.1 1.1
Cell 101.68 114.4 138.58 65.78 88.5 89.91

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/197/RicA1.###

Images:

1 - 180

Beam:

(112.38, 111.95) -> (112.38, 111.98)

Distance:

200.0 -> 200.86

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.23 6.05 2.23
Low resolution limit 53.02 53.04 2.27
Completeness 99.2 99.2 99.7
Multiplicity 3.7 3.5 3.7
I/sigma 7.6 24.1 1.1
Rmerge 0.12 0.026 1.136
CC half 0.995 0.998 0.565
Anomalous completeness 92.1 93.8 91.0
Anomalous multiplicity 2.0 2.0 2.0
Cell 101.72 57.23 126.45 90.0 91.59 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/197/RicA1.###

Images:

1 - 180

Beam:

(112.38, 111.95) -> (112.38, 112.06)

Distance:

200.0 -> 199.89

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.09 5.67 2.09
Low resolution limit 63.11 63.14 2.12
Completeness 99.6 99.9 94.7
Multiplicity 3.7 3.6 3.7
I/sigma 3.1 6.8 1.1
Rmerge 0.36 0.098 1.091
CC half 0.848 0.908 0.42
Anomalous completeness 97.2 99.2 91.2
Anomalous multiplicity 1.9 1.9 1.9
Cell 101.601 57.153 126.274 90.0 91.623 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.