X-Ray Diffraction data from Brucella abortus RicA protein, source of 4N27 structure

Data DOI: 10.15785/SBGRID/197 | ID: 197

Publication DOI: 10.1021/bi401373r

4N27 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Crosson Laboratory, University of Chicago

Release Date: Nov. 24, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/197

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/197 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/197 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/197 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/197 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 197 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Brucella abortus RicA protein

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

beamline 21-ID-F (LS-CAT), Advanced Photon Source, Argonne, Illinois

Data Creation Date:

May 30, 2013

Related Datasets:

None


Cite this Dataset

Herrou, J; Crosson, S. 2015. "X-Ray Diffraction data for: Brucella abortus RicA protein. PDB Code 4N27", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/197.

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Dataset Description

Diffraction data from this crystal of B. abortus RicA were collected at a temperature of 100 K using a 1° oscillation range.

Project Members

Name Additional Roles Affiliation While Working on the Project
Julien HerrouData CollectorUniversity of Chicago
Sean CrossonData Collector, Depositor, PIUniversity of Chicago

Reprocessing Instructions

Images were collected on a MAR Mosaic 225 detector. Indexed in P21.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/197/RicA1.###

Images:

1 - 180

Beam:

(112.38, 111.95) -> (112.38, 112.0)

Distance:

200.0 -> 202.09

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.28 6.19 2.28
Low resolution limit 52.17 52.19 2.32
Completeness 97.5 98.8 96.7
Multiplicity 2.0 2.0 2.0
I/sigma 3.2 8.1 0.7
Rmerge 0.386 0.067 4.258
CC half 0.967 0.992 0.209
Anomalous completeness 83.3 91.0 78.5
Anomalous multiplicity 1.1 1.1 1.1
Cell 101.69 114.42 138.6 65.78 88.5 89.91

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/197/RicA1.###

Images:

1 - 180

Beam:

(112.38, 111.95) -> (112.38, 111.99)

Distance:

200.0 -> 200.86

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.11 5.73 2.11
Low resolution limit 53.03 53.04 2.15
Completeness 99.0 99.1 98.9
Multiplicity 3.7 3.5 3.8
I/sigma 6.6 23.5 0.8
Rmerge 0.143 0.027 1.731
CC half 0.994 0.998 0.327
Anomalous completeness 92.2 93.2 94.1
Anomalous multiplicity 2.0 2.0 2.0
Cell 101.74 57.24 126.46 90.0 91.59 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/197/RicA1.###

Images:

1 - 180

Beam:

(112.38, 111.95) -> (112.38, 112.07)

Distance:

200.0 -> 200.16

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.89 7.84 2.89
Low resolution limit 80.28 80.31 2.94
Completeness 99.9 100.0 97.8
Multiplicity 3.8 3.6 3.9
I/sigma 12.6 56.6 2.4
Rmerge 0.112 0.04 0.27
CC half 0.979 0.968 0.921
Anomalous completeness 98.9 99.9 96.5
Anomalous multiplicity 2.0 2.0 2.0
Cell 101.663 57.175 126.325 90.0 91.617 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.