X-Ray Diffraction data from Prefusion-stabilized RSV F Ectodomain (PR-DM), source of 5C69 structure

Data DOI: 10.15785/SBGRID/191 | ID: 191

Publication DOI: 10.1038/ncomms9143

5C69 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

McLellan Laboratory, University of Texas at Austin

Release Date: Oct. 30, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/191

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/191 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/191 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/191 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/191 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 191 ; shasum -c files.sha'

Storage requirements: 1.8G

Biological Sample:

Prefusion-stabilized RSV F Ectodomain (PR-DM)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

19-ID, SBC, APS, Chicago, IL

Data Creation Date:

Nov. 11, 2014

Related Datasets:

None


Cite this Dataset

McLellan, JS. 2015. "X-Ray Diffraction data for: Prefusion-stabilized RSV F Ectodomain (PR-DM). PDB Code 5C69", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/191.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Jason S McLellanData Collector, DepositorGeisel School of Medicine at Dartmouth
Jason McLellanPIUniversity of Texas at Austin

Reprocessing Instructions

Beam center, as defined in the image headers, needs to be corrected to X=153.1 Y=162.6 Processing with iMosflm will require "Reverse direction of spindle rotation" to be checked

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97924

Sweep:

SWEEP1

Files:

/programs/datagrid/191/P5P14_CC_X1.####.img

Images:

1 - 100

Beam:

(154.5, 159.98) -> (154.5, 162.45)

Distance:

292.16 -> 291.64

Spacegroup:

P 43 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.27 6.16 2.27
Low resolution limit 48.42 48.43 2.31
Completeness 100.0 100.0 100.0
Multiplicity 12.0 10.7 12.2
I/sigma 18.0 58.4 1.2
Rmerge 0.096 0.024 2.066
CC half 0.999 1.0 0.436
Anomalous completeness 100.0 99.9 99.9
Anomalous multiplicity 6.4 6.4 6.4
Cell 167.72 167.72 167.72 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97924

Sweep:

SWEEP1

Files:

/programs/datagrid/191/P5P14_CC_X1.####.img

Images:

1 - 100

Beam:

(154.5, 159.98) -> (154.5, 162.45)

Distance:

292.16 -> 291.8

Spacegroup:

P 43 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.24 6.08 2.24
Low resolution limit 53.04 53.05 2.28
Completeness 100.0 100.0 100.0
Multiplicity 12.0 10.7 12.3
I/sigma 17.4 57.8 1.1
Rmerge 0.1 0.024 2.214
CC half 0.999 1.0 0.424
Anomalous completeness 100.0 99.9 100.0
Anomalous multiplicity 6.4 6.4 6.4
Cell 167.72 167.72 167.72 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97924

Sweep:

SWEEP1

Files:

/programs/datagrid/191/P5P14_CC_X1.####.img

Images:

1 - 100

Beam:

(154.5, 159.98) -> (154.5, 162.45)

Distance:

292.16 -> 291.82

Spacegroup:

P 43 3 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.27 6.16 2.27
Low resolution limit 53.02 53.03 2.31
Completeness 100.0 100.0 100.0
Multiplicity 11.8 10.7 12.1
I/sigma 16.2 51.8 1.2
Rmerge 0.098 0.025 2.105
CC half 0.999 1.0 0.6
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 6.3 6.3 6.3
Cell 167.654 167.654 167.654 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.