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X-Ray Diffraction data from Nup37-Nup120 full-length complex from Schizosaccharomyces pombe, source of 4FHN structure

Data DOI: 10.15785/SBGRID/179 | ID: 179

Publication DOI: 10.1073/pnas.1205151109

4FHN Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Schwartz Laboratory, Massachusetts Institute of Technology

Release Date: Oct. 6, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/179

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/179 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/179 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/179 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/179 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 179 ; shasum -c files.sha'

Storage requirements: 7.1G

Biological Sample:

Nup37-Nup120 full-length complex from Schizosaccharomyces pombe

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24-ID-C

Data Creation Date:

Feb. 19, 2010

Related Datasets:

180


Cite this Dataset

Bilokapic, S; Schwartz, TU. 2015. "X-Ray Diffraction data for: Nup37-Nup120 full-length complex from Schizosaccharomyces pombe. PDB Code 4FHN", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/179.

Download Citation

Dataset Description

native data, collected in two separate spots

Project Members

Name Additional Roles Affiliation While Working on the Project
Thomas U SchwartzData Collector, DepositorMIT
Silvija BilokapicData CollectorMIT
Thomas SchwartzPIMassachusetts Institute of Technology

Reprocessing Instructions

direct beam position for HKL2000: x beam 155.4, y beam 155.9

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.3776

Sweep:

SWEEP2

Files:

/programs/datagrid/179/sb_2_1_2_###.img

Images:

1 - 200

Beam:

(155.63, 156.24) -> (155.63, 156.22)

Distance:

750.0 -> 751.42

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 7.24 19.65 7.24
Low resolution limit 221.37 221.47 7.36
Completeness 100.0 98.0 100.0
Multiplicity 21.5 19.6 12.3
I/sigma 14.8 40.8 0.9
Rmerge 0.168 0.059 3.211
CC half 0.999 0.999 0.323
Anomalous completeness 100.0 99.5 100.0
Anomalous multiplicity 11.8 11.8 11.8
Cell 330.34 330.34 349.47 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.3776

Sweep:

SWEEP2

Files:

/programs/datagrid/179/sb_2_1_2_###.img

Images:

1 - 200

Beam:

(155.63, 156.24) -> (155.63, 156.22)

Distance:

750.0 -> 751.0

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 7.23 19.59 7.23
Low resolution limit 108.13 108.14 7.35
Completeness 100.0 98.8 100.0
Multiplicity 22.0 19.6 12.3
I/sigma 14.8 40.9 0.9
Rmerge 0.172 0.06 3.264
CC half 0.999 0.999 0.307
Anomalous completeness 100.0 99.5 100.0
Anomalous multiplicity 12.0 12.0 12.0
Cell 330.33 330.33 348.96 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.3776

Sweep:

SWEEP2

Files:

/programs/datagrid/179/sb_2_1_2_###.img

Images:

1 - 200

Beam:

(155.63, 156.24) -> (155.63, 156.2)

Distance:

750.0 -> 764.37

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 6.95 18.85 6.95
Low resolution limit 287.27 287.51 7.07
Completeness 99.9 100.0 97.0
Multiplicity 22.6 20.5 22.0
I/sigma 10.3 36.6 0.5
Rmerge 0.199 0.111 3.333
CC half 0.979 0.977 0.355
Anomalous completeness 99.8 100.0 97.0
Anomalous multiplicity 12.3 12.3 12.3
Cell 331.706 331.706 350.533 90.0 90.0 120.0
This dataset is part of the SBDB Pilot Reference Collection, and has processed intensities available from that collection.

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.