X-Ray Diffraction data from Glutamate dehydrogenase from E. coli, source of 4FCC structure

Data DOI: 10.15785/SBGRID/178 | ID: 178

Publication DOI: 10.1073/pnas.1205151109

4FCC Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Schwartz Laboratory, Massachusetts Institute of Technology

Release Date: Oct. 6, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/178

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/178 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/178 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/178 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/178 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 178 ; shasum -c files.sha'

Storage requirements: 6.4G

Biological Sample:

Glutamate dehydrogenase from E. coli

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

NE-CAT

Data Creation Date:

March 17, 2010

Related Datasets:

None


Cite this Dataset

Bilokapic, S; Schwartz, TU. 2015. "X-Ray Diffraction data for: Glutamate dehydrogenase from E. coli. PDB Code 4FCC", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/178.

Download Citation

Dataset Description

native data

Project Members

Name Additional Roles Affiliation While Working on the Project
Thomas U SchwartzData Collector, DepositorMIT
Silvija BilokapicData CollectorMIT
Thomas SchwartzPIMassachusetts Institute of Technology

Reprocessing Instructions

beam center for HKL2000: x beam 157.4, y beam 156.2

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/178/EC3/EC3_1_###.img

Images:

1 - 360

Beam:

(157.69, 156.47) -> (157.69, 156.59)

Distance:

300.0 -> 301.21

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.83 4.97 1.83
Low resolution limit 75.95 76.01 1.86
Completeness 93.3 99.7 61.6
Multiplicity 6.8 6.9 4.7
I/sigma 6.0 10.5 1.0
Rmerge 0.188 0.139 1.416
CC half 0.987 0.986 0.334
Anomalous completeness 92.6 98.8 60.0
Anomalous multiplicity 3.5 3.5 3.5
Cell 105.189 150.116 176.087 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/178/EC3/EC3_1_###.img

Images:

1 - 360

Beam:

(157.69, 156.47) -> (157.69, 156.53)

Distance:

300.0 -> 301.32

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.91 5.18 1.91
Low resolution limit 52.6 52.61 1.94
Completeness 97.3 100.0 76.6
Multiplicity 7.1 6.9 5.3
I/sigma 6.7 12.3 1.0
Rmerge 0.163 0.117 1.297
CC half 0.991 0.99 0.405
Anomalous completeness 96.8 99.7 75.2
Anomalous multiplicity 3.7 3.7 3.7
Cell 150.31 176.34 105.19 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/178/EC3/EC3_1_###.img

Images:

1 - 360

Beam:

(157.69, 156.47) -> (157.69, 156.53)

Distance:

300.0 -> 301.41

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.91 5.18 1.91
Low resolution limit 114.39 114.6 1.94
Completeness 97.3 100.0 76.6
Multiplicity 7.1 6.9 5.3
I/sigma 6.7 12.3 1.0
Rmerge 0.163 0.116 1.3
CC half 0.991 0.991 0.404
Anomalous completeness 96.8 99.7 75.2
Anomalous multiplicity 3.7 3.7 3.7
Cell 150.31 176.34 105.19 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/178/EC3/EC3_1_###.img

Images:

1 - 360

Beam:

(157.69, 156.47) -> (157.69, 156.51)

Distance:

300.0 -> 301.58

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.88 7.82 2.88
Low resolution limit 105.21 105.28 2.93
Completeness 82.9 99.4 0.8
Multiplicity 5.2 5.6 1.0
I/sigma 8.6 9.6 3.6
Rmerge 0.137 0.122 0.0
CC half 0.976 0.985 0.0
Anomalous completeness 72.3 98.5 0.0
Anomalous multiplicity 2.9 2.9 2.9
Cell 105.213 150.333 176.413 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.