X-Ray Diffraction data from Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA), source of 4YCZ structure

Data DOI: 10.15785/SBGRID/175 | ID: 175

Publication DOI: 10.1038/nsmb.2998

4YCZ Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Schwartz Laboratory, Massachusetts Institute of Technology

Release Date: Sept. 25, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/175

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/175 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/175 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/175 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/175 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 175 ; shasum -c files.sha'

Storage requirements: 8.4G

Biological Sample:

Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

beamline 24-ID-C, NE-CAT, APS, Chicago, IL

Data Creation Date:

Feb. 22, 2014

Related Datasets:

None


Cite this Dataset

Knockenhauer, KE; Kelley, K; Schwartz, T. 2015. "X-Ray Diffraction data for: Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA). PDB Code 4YCZ", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/175.

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Dataset Description

SeMet dataset, Nup85 derivatized only

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin E KnockenhauerData Collector, DepositorGraduate Student
Kotaro KelleyData CollectorGraduate Student
Thomas SchwartzPIMassachusetts Institute of Technology

Reprocessing Instructions

Pilatus 6M-F detector, Space group C2

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9789

Sweep:

SWEEP1

Files:

/programs/datagrid/175/hse85_3_15_1_####.cbf

Images:

1 - 1440

Beam:

(216.67, 220.51) -> (216.67, 220.58)

Distance:

750.0 -> 750.18

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.27 11.59 4.27
Low resolution limit 162.11 162.23 4.34
Completeness 90.6 91.6 93.3
Multiplicity 6.9 7.0 7.0
I/sigma 4.6 17.0 1.1
Rmerge 0.382 0.057 1.886
CC half 0.99 0.994 0.379
Anomalous completeness 82.5 83.5 86.3
Anomalous multiplicity 3.6 3.6 3.6
Cell 104.799 117.53 169.558 82.316 72.955 63.75

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9789

Sweep:

SWEEP1

Files:

/programs/datagrid/175/hse85_3_15_1_####.cbf

Images:

1 - 1440

Beam:

(216.67, 220.51) -> (216.67, 220.46)

Distance:

750.0 -> 759.54

Spacegroup:

C 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 5.33 14.4 5.33
Low resolution limit 57.79 57.79 5.42
Completeness 99.5 98.5 100.0
Multiplicity 13.8 13.0 14.4
I/sigma 17.9 48.0 4.3
Rmerge 0.113 0.041 0.751
CC half 0.999 0.999 0.943
Anomalous completeness 99.0 98.0 99.4
Anomalous multiplicity 7.0 7.0 7.0
Cell 105.45 212.5 170.15 90.0 107.36 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9789

Sweep:

SWEEP1

Files:

/programs/datagrid/175/hse85_3_15_1_####.cbf

Images:

1 - 1440

Beam:

(216.67, 220.51) -> (216.67, 220.46)

Distance:

750.0 -> 753.93

Spacegroup:

C 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.44 12.04 4.44
Low resolution limit 90.43 90.45 4.52
Completeness 99.4 98.8 99.6
Multiplicity 13.7 13.2 14.3
I/sigma 11.7 46.4 2.3
Rmerge 0.177 0.043 1.47
CC half 0.998 0.999 0.819
Anomalous completeness 98.9 98.5 99.6
Anomalous multiplicity 7.0 7.0 7.0
Cell 105.44 212.5 170.14 90.0 107.36 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9789

Sweep:

SWEEP1

Files:

/programs/datagrid/175/hse85_3_15_1_####.cbf

Images:

1 - 1440

Beam:

(216.67, 220.51) -> (216.67, 220.45)

Distance:

750.0 -> 759.5

Spacegroup:

C 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.89 10.56 3.89
Low resolution limit 106.57 106.61 3.96
Completeness 98.4 99.1 82.8
Multiplicity 13.4 13.7 13.6
I/sigma 7.1 29.2 1.1
Rmerge 0.307 0.054 4.094
CC half 0.998 0.999 0.604
Anomalous completeness 97.3 98.3 82.1
Anomalous multiplicity 6.8 6.8 6.8
Cell 105.581 213.142 170.964 90.0 107.261 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.