X-Ray Diffraction data from Human GTPase KRAS Q61L bound to GDP, source of 4WA7 structure

Data DOI: 10.15785/SBGRID/161 | ID: 161

Publication DOI: 10.1158/1541-7786.MCR-15-0203

4WA7 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Westover Laboratory, UT Southwestern Medical Center

Release Date: Aug. 28, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/161

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/161 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/161 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/161 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/161 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 161 ; shasum -c files.sha'

Storage requirements: 4.6G

Biological Sample:

Human GTPase KRAS Q61L bound to GDP

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

APS Beamline 19-ID

Data Creation Date:

Feb. 14, 2014

Related Datasets:

None


Cite this Dataset

Hunter, JC; Westover, KD; Westover, K. 2015. "X-Ray Diffraction data for: Human GTPase KRAS Q61L bound to GDP. PDB Code 4WA7", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/161.

Download Citation

Dataset Description

X-Ray diffraction data collected from a crystal of oncogenic mutant of human GTPase KRAS Q61L.

Project Members

Name Additional Roles Affiliation While Working on the Project
John C HunterData Collector, DepositorUT Southwestern Medical Center at Dallas
Kenneth D WestoverUT Southwestern Medical Center at Dallas
Ken WestoverPIUT Southwestern Medical Center

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97915

Sweep:

SWEEP1

Files:

/programs/datagrid/161/Q61L-11b-final.####.img

Images:

1 - 261

Beam:

(154.5, 159.98) -> (154.5, 162.53)

Distance:

274.44 -> 274.58

Spacegroup:

P 63

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.85 5.02 1.85
Low resolution limit 35.69 35.7 1.88
Completeness 100.0 100.0 100.0
Multiplicity 7.8 7.3 6.2
I/sigma 15.5 42.3 0.9
Rmerge 0.061 0.035 1.762
CC half 0.994 0.984 0.408
Anomalous completeness 99.9 99.8 99.4
Anomalous multiplicity 4.0 4.0 4.0
Cell 82.43 82.43 40.7 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97915

Sweep:

SWEEP1

Files:

/programs/datagrid/161/Q61L-11b-final.####.img

Images:

1 - 261

Beam:

(154.5, 159.98) -> (154.5, 162.53)

Distance:

274.44 -> 274.52

Spacegroup:

P 63

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.86 5.05 1.86
Low resolution limit 41.22 41.23 1.89
Completeness 100.0 100.0 99.8
Multiplicity 7.8 7.3 6.5
I/sigma 15.7 42.3 1.0
Rmerge 0.06 0.035 1.667
CC half 0.994 0.985 0.375
Anomalous completeness 99.9 99.8 99.1
Anomalous multiplicity 4.0 4.0 4.0
Cell 82.43 82.43 40.7 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97915

Sweep:

SWEEP1

Files:

/programs/datagrid/161/Q61L-11b-final.####.img

Images:

1 - 261

Beam:

(154.5, 159.98) -> (154.5, 162.53)

Distance:

274.44 -> 273.78

Spacegroup:

P 63

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.95 5.28 1.95
Low resolution limit 35.3 35.3 1.98
Completeness 100.0 100.0 98.8
Multiplicity 7.8 7.5 7.5
I/sigma 9.8 20.6 1.5
Rmerge 0.091 0.058 1.171
CC half 0.992 0.983 0.776
Anomalous completeness 100.0 100.0 98.7
Anomalous multiplicity 4.0 4.0 4.0
Cell 82.254 82.254 40.634 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.