X-Ray Diffraction data from Human GTPase KRAS G12R bound to GDP, source of 4QL3 structure

Data DOI: 10.15785/SBGRID/160 | ID: 160

Publication DOI: 10.1158/1541-7786.MCR-15-0203

4QL3 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Westover Laboratory, UT Southwestern Medical Center

Release Date: Aug. 21, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/160

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/160 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/160 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/160 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/160 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 160 ; shasum -c files.sha'

Storage requirements: 4.6G

Biological Sample:

Human GTPase KRAS G12R bound to GDP

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

APS Beamline 19-ID

Data Creation Date:

Feb. 19, 2014

Related Datasets:

None


Cite this Dataset

Hunter, JC; Westover, KD; Westover, K. 2015. "X-Ray Diffraction data for: Human GTPase KRAS G12R bound to GDP. PDB Code 4QL3", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/160.

Download Citation

Dataset Description

Diffraction Images from native data set collected from a crystal of GDP-bound G12R Oncogenic Mutant of Human GTPase KRas

Project Members

Name Additional Roles Affiliation While Working on the Project
John C HunterData Collector, DepositorUT Southwestern Medical Center at Dallas
Kenneth D WestoverUT Southwestern Medical Center at Dallas
Ken WestoverPIUT Southwestern Medical Center

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97924

Sweep:

SWEEP1

Files:

/programs/datagrid/160/G12R.####.img

Images:

2 - 261

Beam:

(154.5, 159.98) -> (154.5, 159.25)

Distance:

115.07 -> 105.21

Spacegroup:

P 4 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.69 4.48 1.69
Low resolution limit 10.62 10.62 1.72
Completeness 99.5 98.6 99.7
Multiplicity 7.3 5.0 8.5
I/sigma 1.9 1.6 1.2
Rmerge 0.573 0.529 0.823
CC half 0.628 0.531 0.642
Anomalous completeness 97.2 82.4 99.3
Anomalous multiplicity 4.1 4.1 4.1
Cell 36.42 36.42 87.59 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97924

Sweep:

SWEEP1

Files:

/programs/datagrid/160/G12R.####.img

Images:

2 - 261

Beam:

(154.5, 159.98) -> (154.5, 162.22)

Distance:

115.07 -> 115.35

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.0 2.71 1.0
Low resolution limit 45.96 46.01 1.02
Completeness 90.0 100.0 27.8
Multiplicity 4.6 5.0 1.2
I/sigma 12.2 26.8 1.2
Rmerge 0.071 0.047 3.284
CC half 0.993 0.993 0.234
Anomalous completeness 84.9 99.9 4.8
Anomalous multiplicity 2.5 2.5 2.5
Cell 39.173 40.923 91.919 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.