X-Ray Diffraction data from Benzylsuccinate synthase alpha-gamma complex with fumarate bound, source of 5BWD structure

Data DOI: 10.15785/SBGRID/148 | ID: 148

Publication DOI: 10.1074/JBC.M115.670737

5BWD Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Drennan Laboratory, Massachusetts Institute of Technology

Release Date: April 15, 2016

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/148

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/148 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/148 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/148 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/148 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 148 ; shasum -c files.sha'

Storage requirements: 1.5G

Biological Sample:

Benzylsuccinate synthase alpha-gamma complex with fumarate bound

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

NSLS X26C, Brookhaven, NY

Data Creation Date:

July 21, 2011

Related Datasets:

None


Cite this Dataset

Funk, MA; Drennan, C. 2016. "X-Ray Diffraction data for: Benzylsuccinate synthase alpha-gamma complex with fumarate bound. PDB Code 5BWD", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/148.

Download Citation

Dataset Description

Native dataset; data are strongly anisotropic along one axis.

Project Members

Name Additional Roles Affiliation While Working on the Project
Michael A FunkData Collector, DepositorDrennan Lab
Catherine DrennanPIMassachusetts Institute of Technology

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/148/MAF120-3_13_###.img

Images:

1 - 80

Beam:

(157.0, 157.0) -> (157.0, 154.77)

Distance:

325.0 -> 325.92

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.03 5.51 2.03
Low resolution limit 52.98 53.0 2.07
Completeness 98.6 99.9 90.8
Multiplicity 5.9 5.9 3.3
I/sigma 14.3 42.0 1.2
Rmerge 0.066 0.028 0.855
CC half 0.998 0.999 0.628
Anomalous completeness 96.4 98.5 81.1
Anomalous multiplicity 3.1 3.1 3.1
Cell 154.92 154.92 82.23 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/148/MAF120-3_13_###.img

Images:

1 - 80

Beam:

(157.0, 157.0) -> (157.0, 154.77)

Distance:

325.0 -> 325.89

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.01 5.45 2.01
Low resolution limit 52.98 53.0 2.04
Completeness 98.3 99.8 88.8
Multiplicity 5.8 5.9 3.1
I/sigma 13.9 41.8 1.0
Rmerge 0.067 0.028 0.928
CC half 0.998 0.999 0.527
Anomalous completeness 95.8 98.4 77.8
Anomalous multiplicity 3.1 3.1 3.1
Cell 154.92 154.92 82.23 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/148/MAF120-3_13_###.img

Images:

1 - 80

Beam:

(157.0, 157.0) -> (157.0, 154.75)

Distance:

325.0 -> 326.19

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.03 5.51 2.03
Low resolution limit 49.04 49.05 2.07
Completeness 98.6 99.7 88.8
Multiplicity 5.7 5.9 2.8
I/sigma 9.5 25.9 1.0
Rmerge 0.085 0.033 0.848
CC half 0.997 0.998 0.555
Anomalous completeness 95.9 99.6 68.8
Anomalous multiplicity 3.0 3.0 3.0
Cell 155.062 155.062 82.276 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.