X-Ray Diffraction data from Escherichia coli DOS, source of 1V9Y structure

Data DOI: 10.15785/SBGRID/138 | ID: 138

Publication DOI: 10.1074/jbc.M314199200

1V9Y Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Raman Laboratory, University of Maryland

Release Date: June 5, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/138

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/138 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/138 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/138 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/138 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 138 ; shasum -c files.sha'

Storage requirements: 386M

Biological Sample:

Escherichia coli DOS

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

BL 9-2, Stanford Synchrotron Radiation Laboratory

Data Creation Date:

Jan. 4, 2003

Related Datasets:

None


Cite this Dataset

Lee, D; Raman, CS. 2015. "X-Ray Diffraction data for: Escherichia coli DOS. PDB Code 1V9Y", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/138.

Download Citation

Dataset Description

High resolution native X-ray diffraction obtained in two passes. Merging the two datasets yields useable data to 1.6 A resolution.

Project Members

Name Additional Roles Affiliation While Working on the Project
C S RamanData Collector, DepositorUniversity of Texas Medical School
Dong-Sun LeeData CollectorUniversity of Texas Medical School
C S RamanPIUniversity of Maryland

Reprocessing Instructions

XDS/HKL2000/MOSFLM will handle this just fine.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.08

Sweep:

SWEEP2

Files:

/programs/datagrid/138/doslow_###.mar1200

Images:

1 - 120

Beam:

(90.0, 90.0) -> (90.0, 90.94)

Distance:

100.0 -> 98.73

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.6 4.33 1.6
Low resolution limit 23.25 23.25 1.63
Completeness 89.3 100.0 97.8
Multiplicity 6.0 7.8 4.8
I/sigma 5.6 12.0 1.2
Rmerge 0.18 0.095 1.44
CC half 0.993 0.995 0.525
Anomalous completeness 87.5 98.8 92.6
Anomalous multiplicity 3.2 3.2 3.2
Cell 44.81 67.79 81.58 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.08

Sweep:

SWEEP2

Files:

/programs/datagrid/138/doslow_###.mar1200

Images:

1 - 120

Beam:

(90.0, 90.0) -> (90.0, 90.94)

Distance:

100.0 -> 99.5

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.61 4.36 1.61
Low resolution limit 23.25 23.25 1.64
Completeness 89.5 100.0 82.5
Multiplicity 5.6 7.9 4.8
I/sigma 5.1 10.2 1.3
Rmerge 0.153 0.091 1.443
CC half 0.994 0.995 0.581
Anomalous completeness 87.5 98.8 78.2
Anomalous multiplicity 3.0 3.0 3.0
Cell 44.81 67.79 81.58 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.08

Sweep:

SWEEP2

Files:

/programs/datagrid/138/doslow_###.mar1200

Images:

1 - 120

Beam:

(90.0, 90.0) -> (90.0, 90.94)

Distance:

100.0 -> 98.98

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.85 5.02 1.85
Low resolution limit 27.34 27.35 1.89
Completeness 84.6 79.4 73.4
Multiplicity 2.1 2.6 1.5
I/sigma 1.1 1.3 0.9
Rmerge 0.49 0.378 0.335
CC half 0.65 0.764 0.302
Anomalous completeness 56.2 65.7 33.8
Anomalous multiplicity 1.3 1.3 1.3
Cell 45.449 68.424 82.254 89.909 89.958 89.957

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.