X-Ray Diffraction data from Delta25/R222A/K223A mutant of Mg channel CorA from Thermotoga maritima in the presence of Mg++-ions, source of 4EED structure

Data DOI: 10.15785/SBGRID/128 | ID: 128

Publication DOI: 10.1073/pnas.1209018109

4EED Coordinates: Viewer, PDB, MMDB

Pai Laboratory, University of Toronto

Release Date: 21 May 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/128

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/128 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/128 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/128 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/128 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 128 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Delta25/R222A/K223A mutant of Mg channel CorA from Thermotoga maritima in the presence of Mg++-ions

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

08ID-1 of CMCF at the Canadian Light Source

Data Creation Date:

28 Jan 2010

Related Datasets:

None


Cite this Dataset

Pfoh, R; Pai, EF.. 2015. "X-Ray Diffraction data for: Delta25/R222A/K223A mutant of Mg channel CorA from Thermotoga maritima in the presence of Mg++-ions. PDB Code 4EED", SBGrid Data Bank, V1, http://dx.doi.org/10.15785/SBGRID/128.

Download Citation

Dataset Description

Native dataset of triple mutant of T. maritima CorA in the presence of Mg++ ions

Project Members

Name Additional Roles Affiliation While Working on the Project
Roland PfohData CollectorUniversity Health Network; Ontario Cancer Institute
Emil F. PaiDepositor, PIUniversity of Toronto

Reprocessing Instructions

This dataset was indexed in space group no 19 - P212121 and 180 frames were processed with XDS at 3.9 Å resolution.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/128/1c-mg-room_###.img

Images:

1 - 180

Beam:

(112.5, 109.2) -> (112.5, 109.26)

Distance:

350.0 -> 350.1

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.06 11.01 4.06
Low resolution limit 89.17 89.2 4.13
Completeness 100.0 100.0 100.0
Multiplicity 7.2 6.6 7.1
I/sigma 7.8 29.8 1.3
Rmerge 0.167 0.038 1.658
CC half 0.998 0.999 0.472
Anomalous completeness 99.9 99.7 99.7
Anomalous multiplicity 3.8 3.8 3.8
Cell 121.031 131.892 177.84 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/128/1c-mg-room_###.img

Images:

1 - 180

Beam:

(112.5, 109.2) -> (112.5, 109.24)

Distance:

350.0 -> 350.92

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.52 12.25 4.52
Low resolution limit 89.52 89.53 4.59
Completeness 99.9 100.0 94.9
Multiplicity 7.2 6.5 7.3
I/sigma 6.3 20.0 2.3
Rmerge 0.179 0.041 0.928
CC half 0.997 0.997 0.775
Anomalous completeness 99.7 99.4 94.4
Anomalous multiplicity 3.8 3.8 3.8
Cell 121.543 132.338 179.01 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.