X-Ray Diffraction data from Delta25/R222A/K223A mutant of Mg++ channel CorA from Thermotoga maritima in the presence of Cs+-ions, source of 4EEB structure

Data DOI: 10.15785/SBGRID/127 | ID: 127

Publication DOI: 10.1073/pnas.1209018109

4EEB Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Pai Laboratory, University of Toronto

Release Date: May 21, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/127

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/127 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/127 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/127 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/127 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 127 ; shasum -c files.sha'

Storage requirements: 3.5G

Biological Sample:

Delta25/R222A/K223A mutant of Mg++ channel CorA from Thermotoga maritima in the presence of Cs+-ions

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

08ID-1 of CMCF at the Canadian Light Source

Data Creation Date:

Dec. 8, 2007

Related Datasets:

None


Cite this Dataset

Pfoh, R; Pai, EF.. 2015. "X-Ray Diffraction data for: Delta25/R222A/K223A mutant of Mg++ channel CorA from Thermotoga maritima in the presence of Cs+-ions. PDB Code 4EEB", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/127.

Download Citation

Dataset Description

Native dataset of triple mutant crystallized in the presence of Cs+ and Na+ ions.

Project Members

Name Additional Roles Affiliation While Working on the Project
Roland PfohData CollectorUniversity Health Network; Ontario Cancer Institute
Emil F. PaiDepositor, PIUniversity of Toronto

Reprocessing Instructions

This dataset was indexed in space group no. 18 - P21212 and 192 frames were processed with XDS at 3.8 Å resolution.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97934

Sweep:

SWEEP1

Files:

/programs/datagrid/127/J2.2_1_E0_####.img

Images:

1 - 192

Beam:

(112.57, 111.11) -> (112.57, 110.96)

Distance:

300.0 -> 300.0

Spacegroup:

P 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.81 10.34 3.81
Low resolution limit 95.12 95.15 3.88
Completeness 96.6 88.9 92.8
Multiplicity 3.6 3.8 3.1
I/sigma 6.1 19.5 1.3
Rmerge 0.107 0.035 0.775
CC half 0.995 0.996 0.312
Anomalous completeness 93.6 96.0 83.8
Anomalous multiplicity 2.0 2.0 2.0
Cell 105.688 124.257 218.288 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97934

Sweep:

SWEEP1

Files:

/programs/datagrid/127/J2.2_1_E0_####.img

Images:

1 - 192

Beam:

(112.57, 111.11) -> (112.57, 110.85)

Distance:

300.0 -> 299.93

Spacegroup:

P 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 5.64 15.2 5.64
Low resolution limit 53.91 53.92 5.74
Completeness 96.7 85.8 98.1
Multiplicity 3.9 3.3 4.0
I/sigma 23.0 43.7 6.1
Rmerge 0.027 0.021 0.22
CC half 0.998 0.997 0.945
Anomalous completeness 94.1 83.4 96.0
Anomalous multiplicity 2.1 2.1 2.1
Cell 105.61 124.32 217.83 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97934

Sweep:

SWEEP1

Files:

/programs/datagrid/127/J2.2_1_E0_####.img

Images:

1 - 192

Beam:

(112.57, 111.11) -> (112.57, 110.85)

Distance:

300.0 -> 299.66

Spacegroup:

P 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 5.94 15.99 5.94
Low resolution limit 53.91 53.92 6.04
Completeness 96.5 85.4 99.2
Multiplicity 3.9 3.3 4.0
I/sigma 25.8 44.2 7.6
Rmerge 0.025 0.021 0.162
CC half 0.998 0.996 0.965
Anomalous completeness 94.0 81.5 96.4
Anomalous multiplicity 2.1 2.1 2.1
Cell 105.61 124.32 217.83 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97934

Sweep:

SWEEP1

Files:

/programs/datagrid/127/J2.2_1_E0_####.img

Images:

1 - 192

Beam:

(112.57, 111.11) -> (112.57, 110.91)

Distance:

300.0 -> 300.33

Spacegroup:

P 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.09 11.06 4.09
Low resolution limit 52.77 52.78 4.16
Completeness 97.8 91.5 90.3
Multiplicity 3.9 3.7 3.9
I/sigma 4.4 8.8 1.7
Rmerge 0.106 0.046 0.648
CC half 0.985 0.977 0.553
Anomalous completeness 96.3 96.5 87.2
Anomalous multiplicity 2.1 2.1 2.1
Cell 105.549 124.273 217.909 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.