Data DOI: 10.15785/SBGRID/127 | ID: 127
Publication DOI: 10.1073/pnas.1209018109
4EEB Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Pai Laboratory, University of Toronto
Release Date: 21 May 2015
1. If this dataset is locally available, it should be accessable at /programs/datagrid/127
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/127 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/127 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/127 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/127 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 127 ; shasum -c files.sha'
Storage requirements: 3.5G
Biological Sample:
Delta25/R222A/K223A mutant of Mg++ channel CorA from Thermotoga maritima in the presence of Cs+-ions
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
08ID-1 of CMCF at the Canadian Light Source
Data Creation Date:
8 Dec 2007
Related Datasets:
None
Pfoh, R; Pai, EF.. 2015. "X-Ray Diffraction data for: Delta25/R222A/K223A mutant of Mg++ channel CorA from Thermotoga maritima in the presence of Cs+-ions. PDB Code 4EEB", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/127.
Native dataset of triple mutant crystallized in the presence of Cs+ and Na+ ions.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Roland Pfoh | Data Collector | University Health Network; Ontario Cancer Institute |
| Emil F. Pai | Depositor, PI | University of Toronto |
This dataset was indexed in space group no. 18 - P21212 and 192 frames were processed with XDS at 3.8 Å resolution.
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97934
Sweep:
SWEEP1
Files:
/programs/datagrid/127/J2.2_1_E0_####.img
Images:
1 - 192
Beam:
(112.57, 111.11) -> (112.57, 110.85)
Distance:
300.0 -> 299.79
Spacegroup:
P 2 2 21
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 3.83 | 10.37 | 3.83 |
| Low resolution limit | 53.92 | 53.93 | 3.9 |
| Completeness | 98.6 | 92.7 | 99.8 |
| Multiplicity | 4.0 | 3.6 | 4.0 |
| I/sigma | 10.0 | 43.5 | 1.1 |
| Rmerge | 0.069 | 0.019 | 1.298 |
| CC half | 0.998 | 0.998 | 0.323 |
| Anomalous completeness | 94.8 | 90.9 | 94.5 |
| Anomalous multiplicity | 2.1 | 2.1 | 2.1 |
| Cell | 105.63 | 124.34 | 217.86 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97934
Sweep:
SWEEP1
Files:
/programs/datagrid/127/J2.2_1_E0_####.img
Images:
1 - 192
Beam:
(112.57, 111.11) -> (112.57, 110.84)
Distance:
300.0 -> 299.67
Spacegroup:
P 2 2 21
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 3.82 | 10.35 | 3.82 |
| Low resolution limit | 53.92 | 53.93 | 3.89 |
| Completeness | 98.6 | 92.8 | 99.9 |
| Multiplicity | 4.0 | 3.6 | 4.0 |
| I/sigma | 9.9 | 43.9 | 1.1 |
| Rmerge | 0.07 | 0.018 | 1.389 |
| CC half | 0.998 | 0.998 | 0.299 |
| Anomalous completeness | 94.8 | 90.9 | 94.4 |
| Anomalous multiplicity | 2.1 | 2.1 | 2.1 |
| Cell | 105.63 | 124.34 | 217.87 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.97934
Sweep:
SWEEP1
Files:
/programs/datagrid/127/J2.2_1_E0_####.img
Images:
1 - 192
Beam:
(112.57, 111.11) -> (112.57, 110.92)
Distance:
300.0 -> 300.02
Spacegroup:
P 2 2 2
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 4.22 | 11.44 | 4.22 |
| Low resolution limit | 107.83 | 107.86 | 4.29 |
| Completeness | 98.6 | 93.3 | 98.7 |
| Multiplicity | 4.0 | 3.8 | 3.9 |
| I/sigma | 6.0 | 34.8 | 1.0 |
| Rmerge | 0.12 | 0.091 | 0.476 |
| CC half | 0.918 | 0.809 | 0.176 |
| Anomalous completeness | 98.0 | 99.8 | 96.5 |
| Anomalous multiplicity | 2.1 | 2.1 | 2.1 |
| Cell | 105.491 | 124.139 | 217.618 | 90.0 | 90.0 | 90.0 |
|---|
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
