X-Ray Diffraction data from Caulobacter PhyR sigma-like domain in complex with NepR, source of 3T0Y structure

Data DOI: 10.15785/SBGRID/116 | ID: 116

Publication DOI: 10.1073/pnas.1116887109

3T0Y Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Crosson Laboratory, University of Chicago

Release Date: May 19, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/116

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/116 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/116 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/116 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/116 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 116 ; shasum -c files.sha'

Storage requirements: 3.6G

Biological Sample:

Caulobacter PhyR sigma-like domain in complex with NepR

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

LS-CAT. 21-ID-D

Data Creation Date:

Nov. 24, 2010

Related Datasets:

None


Cite this Dataset

Herrou, J; Crosson, S. 2015. "X-Ray Diffraction data for: Caulobacter PhyR sigma-like domain in complex with NepR. PDB Code 3T0Y", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/116.

Download Citation

Dataset Description

native dataset of PhyR-SL/NepR complex

Project Members

Name Additional Roles Affiliation While Working on the Project
Julien HerrouData CollectorUniversity of Chicago
Sean CrossonData Collector, Depositor, PIUniversity of Chicago

Reprocessing Instructions

Diffraction images were reduced using HKL 2000 suite; crystal was assigned to space group C2221. Cell dimensions a = 75.4 Å, b = 105.3 Å, and c = 97.9 Å. Diffraction from these crystals was moderately anisotropic (Dmin = 2.1 Å along b* and c* and 2.7 Å along a*, where Dmin is the diffraction limit of the crystal). The reflection set was ellipsoidally truncated and anisotropically scaled before refinement.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/116/cryst1.###

Images:

6 - 200

Beam:

(111.39, 111.97) -> (111.39, 111.97)

Distance:

180.0 -> 180.02

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.04 5.54 2.04
Low resolution limit 52.53 52.55 2.08
Completeness 98.9 98.5 98.6
Multiplicity 7.8 7.2 7.9
I/sigma 9.2 29.8 1.4
Rmerge 0.12 0.032 0.97
CC half 0.998 0.998 0.92
Anomalous completeness 99.0 99.2 98.1
Anomalous multiplicity 4.1 4.1 4.1
Cell 76.945 105.062 97.938 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/116/cryst1.###

Images:

6 - 200

Beam:

(111.39, 111.97) -> (111.39, 111.86)

Distance:

180.0 -> 179.57

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.6 7.05 2.6
Low resolution limit 52.47 52.48 2.65
Completeness 99.0 98.7 96.7
Multiplicity 7.8 6.8 8.1
I/sigma 22.7 50.1 5.1
Rmerge 0.044 0.024 0.364
CC half 0.999 0.999 0.983
Anomalous completeness 98.7 97.4 96.7
Anomalous multiplicity 4.1 4.1 4.1
Cell 76.87 105.2 98.24 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/116/cryst1.###

Images:

6 - 200

Beam:

(111.39, 111.97) -> (111.39, 111.86)

Distance:

180.0 -> 179.88

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.48 6.73 2.48
Low resolution limit 52.6 52.61 2.52
Completeness 99.2 99.0 99.4
Multiplicity 7.8 6.8 8.0
I/sigma 20.5 49.6 4.4
Rmerge 0.047 0.024 0.413
CC half 0.999 0.999 0.985
Anomalous completeness 98.9 97.9 99.2
Anomalous multiplicity 4.1 4.1 4.1
Cell 76.87 105.2 98.24 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97872

Sweep:

SWEEP1

Files:

/programs/datagrid/116/cryst1.###

Images:

6 - 200

Beam:

(111.39, 111.97) -> (111.39, 111.92)

Distance:

180.0 -> 180.44

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.11 5.72 2.11
Low resolution limit 52.84 52.85 2.14
Completeness 99.7 99.2 96.1
Multiplicity 8.0 7.3 8.1
I/sigma 9.3 28.2 1.1
Rmerge 0.093 0.03 1.251
CC half 0.998 0.997 0.909
Anomalous completeness 99.6 99.0 95.8
Anomalous multiplicity 4.2 4.2 4.2
Cell 77.435 105.897 98.883 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.