X-Ray Diffraction data from Lin28A/let-7g microRNA complex, source of 3TS2 structure

Data DOI: 10.15785/SBGRID/1 | ID: 1

Publication DOI: 10.1016/j.cell.2011.10.020

3TS2 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Sliz Laboratory, Harvard Medical School

Release Date: April 10, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/1

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/1 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/1 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/1 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/1 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 1 ; shasum -c files.sha'

Storage requirements: 1.6G

Biological Sample:

Lin28A/let-7g microRNA complex

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein, RNA

Collection Facility:

APS 24-ID-C

Data Creation Date:

Feb. 23, 2011

Related Datasets:

None


Cite this Dataset

Nam, Y; Sliz, P. 2015. "X-Ray Diffraction data for: Lin28A/let-7g microRNA complex. PDB Code 3TS2", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/1.

Download Citation

Dataset Description

Native Dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Yunsun NamData CollectorHarvard Medical School
Piotr SlizDepositor, PIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/1/p3_6_1_###.img

Images:

1 - 90

Beam:

(157.69, 156.47) -> (157.69, 156.54)

Distance:

300.0 -> 301.43

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.88 5.1 1.88
Low resolution limit 52.23 52.25 1.91
Completeness 91.3 93.8 64.5
Multiplicity 3.0 3.3 2.1
I/sigma 4.6 7.2 1.0
Rmerge 0.131 0.13 1.374
CC half 0.912 0.892 0.239
Anomalous completeness 79.3 90.2 39.8
Anomalous multiplicity 1.7 1.7 1.7
Cell 45.98 108.991 182.848 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/1/p3_6_1_###.img

Images:

1 - 90

Beam:

(157.69, 156.47) -> (157.69, 156.48)

Distance:

300.0 -> 301.52

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.03 5.51 2.03
Low resolution limit 41.3 41.31 2.07
Completeness 92.2 58.0 96.9
Multiplicity 3.6 3.4 3.3
I/sigma 6.5 4.2 1.2
Rmerge 0.107 0.095 1.189
CC half 0.995 0.995 0.717
Anomalous completeness 88.2 54.1 89.6
Anomalous multiplicity 1.9 1.9 1.9
Cell 46.01 109.16 182.83 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/1/p3_6_1_###.img

Images:

1 - 90

Beam:

(157.69, 156.47) -> (157.69, 156.47)

Distance:

300.0 -> 301.64

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.92 5.21 1.92
Low resolution limit 42.4 42.41 1.95
Completeness 97.0 93.5 83.9
Multiplicity 3.4 3.4 2.4
I/sigma 14.5 33.4 1.0
Rmerge 0.039 0.023 1.102
CC half 0.998 0.996 0.41
Anomalous completeness 90.2 89.7 63.5
Anomalous multiplicity 1.9 1.9 1.9
Cell 46.01 109.16 182.82 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/1/p3_6_1_###.img

Images:

1 - 90

Beam:

(157.69, 156.47) -> (157.69, 156.46)

Distance:

300.0 -> 302.64

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.81 7.23 2.81
Low resolution limit 13.35 13.35 2.86
Completeness 63.8 4.6 1.6
Multiplicity 3.1 1.1 1.0
I/sigma 16.8 14.7 8.3
Rmerge 0.036 0.0 0.0
CC half 0.997 1.0 0.0
Anomalous completeness 54.6 0.4 0.0
Anomalous multiplicity 1.8 1.8 1.8
Cell 46.015 109.196 182.848 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.