X-Ray Diffraction data from Mn-bound D296A mutant Nramp/MntH divalent transition metal transporter from Deinococcus radiodurans, source of 8E6L structure

Data DOI: 10.15785/SBGRID/968 | ID: 968

Publication DOI: 10.7554/eLife.84006

8E6L Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Gaudet Laboratory, Harvard University

Release Date: 2 May 2023

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/968

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/968 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/968 .' (Uppsala University, Sweden)

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3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 968 ; shasum -c files.sha'

Storage requirements: 23G

Biological Sample:

Mn-bound D296A mutant Nramp/MntH divalent transition metal transporter from Deinococcus radiodurans

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

Beamline 23-ID-B with Dectris Eiger-16M pixel detector, NE-CAT, APS, Chicago, IL

Data Creation Date:

20 Sep 2020

Related Datasets:

None


Cite this Dataset

Ray, S; Gaudet, R. 2023. "X-Ray Diffraction data for: Mn-bound D296A mutant Nramp/MntH divalent transition metal transporter from Deinococcus radiodurans. PDB Code 8E6L", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/968.

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Dataset Description

Datasets of Mn-bound D296A was collected from 3 LCP crystals. Image files that start C7run1_17 are from the first crystal, C7run2_19 are from the second crystal and C7run4_20 are from the third crystal.

Project Members

Name Additional Roles Affiliation While Working on the Project
Shamayeeta RayData Collector, DepositorHarvard University
Rachelle GaudetData Collector, PIHarvard University

Reprocessing Instructions

Data was processed using XDS, indexed using POINTLESS in space group P22121, scaled using AIMLESS at 3.11A.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.