X-Ray Diffraction data from S. cerevisiae Csm1-Mam1 complex, source of 4EMC structure

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Data DOI: 10.15785/SBGRID/24 | ID: 24

Publication DOI: 10.1016/j.celrep.2012.05.012

4EMC Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Harrison Laboratory, Harvard Medical School

Release Date: 6 May 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/24

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/24 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/24 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/24 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/24 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 24 ; shasum -c files.sha'

Storage requirements: 721M

Biological Sample:

S. cerevisiae Csm1-Mam1 complex

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Advanced Photon Source BL 24ID-E

Data Creation Date:

21 Apr 2011

Related Datasets:

None


Cite this Dataset

Corbett, KD; Harrison, S. 2015. "X-Ray Diffraction data for: S. cerevisiae Csm1-Mam1 complex. PDB Code 4EMC", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/24.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettData Collector, DepositorHarvard Medical School
Stephen HarrisonPIHarvard Medical School

Reprocessing Instructions

Process to 3.05 Å

Reprocessing Data

About Reprocessing

xia2 -2d

xia2 -3d

xia2 -3dii

xia2 -dials
/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=3d /programs/datagrid//24/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/24/kdc_apr11_d3_1_###.img

Images:

1 - 40

Beam:

(157.19, 156.39) -> (157.19, 156.31)

Distance:

400.0 -> 403.13

Spacegroup:

P 3 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.98 8.08 2.98
Low resolution limit 49.79 49.79 3.03
Completeness 98.3 92.8 99.4
Multiplicity 2.4 2.3 2.5
I/sigma 8.0 19.9 0.8
Rmerge 0.074 0.028 1.303
CC half 0.996 0.996 0.333
Anomalous completeness 70.3 79.8 65.4
Anomalous multiplicity 1.5 1.5 1.5
Cell 102.09 102.09 225.72 90.0 90.0 120.0
/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=3dii /programs/datagrid//24/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/24/kdc_apr11_d3_1_###.img

Images:

1 - 40

Beam:

(157.19, 156.39) -> (157.19, 156.31)

Distance:

400.0 -> 402.73

Spacegroup:

P 3 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.98 8.08 2.98
Low resolution limit 49.79 49.79 3.03
Completeness 98.3 92.8 99.4
Multiplicity 2.4 2.3 2.5
I/sigma 8.0 19.8 0.8
Rmerge 0.074 0.028 1.302
CC half 0.996 0.996 0.335
Anomalous completeness 70.3 79.7 65.3
Anomalous multiplicity 1.5 1.5 1.5
Cell 102.09 102.09 225.72 90.0 90.0 120.0
/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=dials /programs/datagrid//24/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/24/kdc_apr11_d3_1_###.img

Images:

1 - 40

Beam:

(157.19, 156.39) -> (157.19, 156.27)

Distance:

400.0 -> 402.52

Spacegroup:

C 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.87 7.77 2.87
Low resolution limit 47.6 47.61 2.92
Completeness 52.6 45.7 52.5
Multiplicity 1.6 1.8 1.5
I/sigma 9.3 55.8 0.2
Rmerge 0.07 0.039 1.427
CC half 0.98 0.968 0.312
Anomalous completeness 22.7 31.2 17.8
Anomalous multiplicity 1.1 1.1 1.1
Cell 177.213 101.928 226.247 90.0 90.169 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.